工业大麻WRKY家族全基因组鉴定及生物信息学分析  

Genome-Wide Identification and Bioinformatics Analysis of the WRKY Gene Family in Industrial Hemp

作　　者：张元野 张利国[1] 张明[1] 房郁研[1] 郑楠[1] 闫博巍 隋月 ZHANG Yuanye;ZHANG Liguo;ZHANG Ming;FANG Yuyan;ZHENG Nan;YAN Bowei;SUI Yue(Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2024年第10期17-26,共10页Heilongjiang Agricultural Sciences

基　　金：黑龙江省农业科技创新跨越工程——经济作物突破性新品种选育及产业化应用(GX23GG04)。

摘　　要：WRKY转录因子是一类具有多种生物学功能的基因,工业大麻中WRKY家族研究较少。本研究使用拟南芥WRKY家族蛋白序列与工业大麻全基因组进行比对,鉴定到34个WRKY家族成员,不均等分布于9条染色体上,同时可分为GroupⅠ、GroupⅡa~GroupⅡe、GroupⅢ共7个亚族,其中GroupⅠ亚族成员数量最多,数量为11个;CsWRKY15包含的结构域最多、CsWRKY34启动子区域的顺式作用元件最多,二者可能行使着较多生物学功能;工业大麻WRKY家族与拟南芥、水稻、玉米、大豆及大麻自身间共线性分析显示,CsWRKYs存在丰富的直系同源基因进化关系。WRKY transcription factors are a class of genes with multiple biological functions,and the WRKY family in cannabis is rarely studied.We used Arabidopsis WRKY family protein sequences to compare with the whole genome of cannabis,and identified 34 WRKY family members,which were unequally distributed on 9 chromosomes,and could be divided into 7 subfamilies,including GroupⅠ,GroupⅡa-GroupⅡe,and GroupⅢ,among which GroupⅠhad the largest number of members,with 11 members.CsWRKY15 contains the most domains,and CsWRKY34 has the most cisacting elements in the promoter region,so they may have more biological functions.The collinearity analysis between the WRKY family of cannabis and Arabidopsis thaliana,rice,maize,soybean and cannabis itself showed that CsWRKYs had rich phylogenetic relationships.This course lays a data foundation for exploring the biological function of cannabis WRKY family.

关 键 词：工业大麻 WRKY 蛋白理化性质 同源性分析 生物信息学 

分 类 号：S563[农业科学—作物学]