糙叶败酱中獐牙菜苷的高速逆流色谱分离及其抗肿瘤活性研究  

Separation of Sweroside from Patrinia scabra by High-Speed Countercurrent Chromatography and Study on Its Antitumor Activity

作　　者：王梦凡 李彬 辛安琪 马趣环[2] 石晓峰[2] Wang Mengfan;Li Bin;Xin Anqi;Ma Quhuan;Shi Xiaofeng(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Gansu Provincial Academy of Medical Sciences,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省医学科学研究院,甘肃兰州730050

出　　处：《中国野生植物资源》2024年第8期29-35,40,共8页Chinese Wild Plant Resources

基　　金：兰州市人才创新创业项目(2022-3-38);甘肃省自然科学基金项目(3JRRA1802);兰州市科学技术局项目(2020-XG-21)。

摘　　要：目的:采用高速逆流色谱法(HSCCC)对糙叶败酱中的环烯醚萜类化合物獐牙菜苷进行分离纯化,并对其体外抗肿瘤活性进行初步评价。方法:HSCCC的溶剂体系为正丁醇-乙酸乙酯-水(5∶2∶5),上相为固定相,下相为流动相,正进正转,分离温度25℃、转速850 r/min、流动相流速2.0 mL/min、进样浓度10 mg/mL、进样量15 mL、检测波长240 nm,并采用细胞计数试剂盒-8(CCK-8)法检测糙叶败酱对5种不同肿瘤细胞的增殖抑制作用。结果:从糙叶败酱中分离得到了纯度大于92%的獐牙菜苷。獐牙菜苷对人源肝癌细胞HepG2、结肠癌细胞SW480、肺癌细胞A549、人慢性髓原白血病细胞K562和宫颈癌Hela细胞这5种肿瘤细胞的增殖均有较强的抑制作用,且呈现明显的剂量依赖关系和时间依赖关系。结论:本研究为应用HSCCC高效分离纯化糙叶败酱中獐牙菜苷提供了技术支持,也为其抗肿瘤活性研究提供了思路。Objective:To separate and purify the iridoid compound swertiamarin from Patrinia scabra Bunge by high-speed counter-current chromatography(HSCCC),and to preliminarily evaluate its antitumor activity in vitro.Methods:The solvent system of HSCCC was n-butanol-ethyl acetate-water(5∶2∶5),with the upper phase was the stationary phase and the lower phase was the mobile phase.The separation temperature was 25℃,the rotational speed was 850 r/min,the mobile phase flow rate was 2.0 mL/min,the injection concentration was 10 mg/mL,the injection volume was 15 mL,and the detection wavelength was 240 nm.The cell counting kit(CCK-8)method was used to detect the inhibitory effect of swertiamarin on the proliferation of five different kinds of tumor cells.Results:It showed that sweroside with a purity greater than 92%was isolated from P.scabra.Sweroside showed strong inhibitory effect on the proliferation of human liver cancer cells HepG2 cells,colon cancer cells SW480,lung cancer A549 cells,human chronic myeloid leukemia K562 cells,and cervical cancer Hela cells.The antitumor effects of swertiamarin showed obvious dose-dependent and time-dependent relationship.Conclusion:The study provided technical support for the application of HSCCC to efficiently isolate and purify swertiamarin from P.scabra,and provided ideas for the research of its antitumor activity.

关 键 词：糙叶败酱 高速逆流色谱 獐牙菜苷 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]