中药组方预防多重耐药鸡柔嫩艾美耳球虫感染的效果探究  

Preventive Effect of a Self-Made Herbal Formula on Infection of Eimeria tenella with Multidrug Resistance in Chickens

作　　者：田伟 陈建辉 陈慧敏 黄俊杰 郑淑媚 槐玉英 李金贵 刘明江 TIAN Wei;CHEN Jianhui;CHEN Huimin;HUANG Junjie;ZHENG Shumei;HUAI Yuying;LI Jingui;LIU Mingjiang(College of Veterinary Medicine(Institute of Comparative Medicine),Yangzhou University,Yangzhou,Jiangsu 225009;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009)

机构地区：[1]扬州大学兽医学院(比较医学研究院),江苏扬州225009 [2]江苏省高校动物重要疫病与人兽共患病防控协同中心,江苏扬州225009

出　　处：《中国家禽》2024年第10期73-80,共8页China Poultry

基　　金：江苏省高校优势学科建设工程项目(PAPD);扬州大学教改课题项目(YZUJX2021-C18)。

摘　　要：研究旨在探究自拟中药组方“香芪汤”对田间分离的多重耐药鸡柔嫩艾美耳球虫(E.tenella)感染的预防效果研究。72只10日龄雄性黄羽肉鸡随机分为6组,分别为空白对照组、感染组、中药预防组、中药防治组、地克珠利预防组和中药对照组。除空白对照组、中药对照组外,其余各组鸡于15日龄时经口灌服5×10~4个柔嫩艾美耳球虫孢子化卵囊(记为0 h);中药预防组、地克珠利预防组和中药对照组感染前120 h连续在饮水中添加相应药物至0 h,中药防治组感染前48 h在饮水中添加相应药物至感染后72 h。感染后192 h剖杀各组鸡,采集盲肠、脾脏、肝脏组织。记录并统计各组鸡脏器指数、相对增重率、盲肠病变记分、卵囊值和存活率,计算抗球虫指数(ACI);利用组织切片和扫描电镜观察盲肠损伤,试剂盒检测盲肠组织中炎症、氧化应激相关因子水平。结果显示:与感染组相比,中药预防组、中药防治组ACI值均显著升高(P<0.05),肝脏、脾脏指数均显著降低(P<0.05),盲肠黏膜组织结构损伤均明显减轻,盲肠组织中促炎因子(肿瘤坏死因子-α、白细胞介素-1β)、丙二醛含量显著降低(P<0.05),抑炎因子白细胞介素-10含量、抗氧化酶(超氧化物歧化酶、谷胱甘肽过氧化物酶)活性显著升高(P<0.05)。结果表明“,香芪汤”对多重耐药鸡柔嫩艾美耳球虫株感染具有良好的预防效果。This study was aimed to explore the preventive and therapeutic effect,as well as the potential mechanism of a self-made traditional Chinese medicine formula called"Xiangqi Decoction"on a coccidiosis model infected with a multidrug resistant strain of Eimeria tenella.Seventy-two 10-day-age male yellow-feather broiler chicks were randomly divided into 6 groups based on body weight:the blank control group,the infected group,the traditional Chinese medicine prevention group,the traditional Chinese medicine prevention and treatment group,the diclazuril prevention group and the traditional Chinese medicine control group.Except for the blank control group and the traditional Chinese medicine group,each bird in all other groups were orally inoculated with 5×104 sporulated oocysts(0 h).The traditional Chinese medicine prevention group,diclazuril prevention group,and traditional Chinese medicine group were administered medicines continuously in their drinking water for 120 h prior to oocyst inoculation.In the traditional Chinese medicine prevention and treatment group,the medicine was administered from 48 h before oocyst inoculation until 72 h post inoculation(PI).All birds were sacrificed,and the ceca,spleens and livers were collected at 192 h(PI).The relative body weight gain,cecal lesion score,oocyst value,organ index and survival rate of chickens in each group were recorded,and the anticoccidial index(ACI)was calculated.The histological damage of cecum was observed using histological sections and scanning electron microscopy.Additionally,the levels of inflammation and oxidative stress-related factors in the cecum tissue were detected by commercial kits.The results showed as follows:Compared with the infected group,ACI in the traditional Chinese medicine prevention group and the traditional Chinese medicine prevention/treatment group was significantly improved(P<0.05);The liver and spleen indexes,cecal mucosal structural injury,pro-inflammatory cytokines(tumor necrosis factor-α,interleukin-1β)and malondialdehyde were

关 键 词：柔嫩艾美耳球虫 中药组方 耐药性 抗球虫指数 盲肠损伤 

分 类 号：S852.7[农业科学—基础兽医学]