等渗PEG与NaCl胁迫对梭梭种子萌发和幼苗生长抑制研究  

Study on Mechanism of Isotonic PEG and NaCl Stress on Seed Germination and Seedling Growth of Haloxylon ammodendron

作　　者：马桃桃 郭晔红[1] 安芳芳 杨佳龙 李梅英[2] 王清波 Ma Taotao;Guo Yehong;An Fangfang;Yang Jialong;Li Meiying;Wang Qingbo(State Key Lab.of Aridland Crop Science,College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;Gansu Endangered Animal Protection Center of the State Forestry Administration,Wuwei 730000,China;Pharmacy Department of Shuangyashan Traditional Chinese Medicine Hospital in Heilongjiang Province,Shuangyashan 155100,China)

机构地区：[1]甘肃农业大学农学院干旱生境作物学国家重点实验室,甘肃兰州730070 [2]国家林业局甘肃濒危动物保护中心,甘肃武威733000 [3]黑龙江双鸭山中医院药剂科,黑龙江双鸭山155100

出　　处：《中国野生植物资源》2024年第9期49-56,共8页Chinese Wild Plant Resources

基　　金：国家自然科学基金项目(31860349);甘肃省科技计划项目(18YFlNA072)。

摘　　要：目的:探讨不同干旱类型对梭梭种子萌发及早期幼苗生长的抑制程度,以期为干旱环境条件下梭梭种苗的高效培育提供科学支撑。方法:以梭梭种子为材料,采用PEG模拟土壤干旱,NaCl模拟生理干旱,通过测定发芽率、发芽势、发芽指数、幼苗活力指数、幼苗长和幼苗根长、根冠比等指标,研究不同等渗梯度(0、-0.46、-1.38、-2.30、-3.23 Mpa)下2种胁迫对梭梭种子萌发及早期幼苗生长的影响。结果:高浓度PEG胁迫(-1.38~-3.23 Mpa)使梭梭种子发芽率、发芽势、发芽指数、幼苗活力指数、幼苗长和根长显著降低,表明中度土壤干旱即可抑制梭梭种子萌发及早期幼苗生长。重度NaCl胁迫(-3.23 Mpa)显著降低发芽率、发芽势和发芽指数,轻度NaCl胁迫(-0.46 Mpa)下幼苗活力指数和根冠比显著高于对照,表明重度生理干旱明显抑制梭梭种子萌发,轻度生理干旱促进梭梭早期幼苗生长。NaCl胁迫比PEG胁迫有更高的发芽率、发芽势、发芽指数、幼苗活力指数、幼苗长和根冠比。结论:土壤干旱和生理干旱显著抑制了梭梭种子萌发及幼苗生长,但土壤干旱抑制作用更强。梭梭更适宜在土壤水势不低于-2.25 Mpa的干旱盐碱地区播种育苗。Objective:To investigate the inhibition degree of different drought types on seed germination and early seedling growth of Haloxylon ammodendron,with a view to providing scientific support for the efficient cultivation of H.ammodendron seedlings under arid environmental conditions.Methods:Seeds of H.ammodendron were used as materials,PEG was used to simulate soil drought,and NaCl was used to simulate physiological drought.The effects of the two stresses under different isotonic gradients(0,-0.46,-1.38,-2.30,-3.23 Mpa)on the seed germination and early seedling growth of H.ammodendron were studied by determining germination rate,germination potential,germination index,seedling vitality index,seedling growth and seedling root length,root-to-head ratio and other indicators.Results:High concentrations of PEG stress significantly reduced the germination rate,germination potential,germination index,seedling vitality index,seedling length and root length of H.ammodendron,indicating that moderate soil drought could inhibit seed germination and early seedling growth of H.ammodendron.Severe NaCl stress(-3.23 Mpa)significantly reduced germination rate,germination potential and germination index,while seedling vitality index and root-to-head ratio were significantly higher than the control under mild NaCl stress(-0.46 Mpa),indicating that severe physiological drought significantly inhibited the germination of H.ammodendron seeds,while mild physiological drought promoted the growth of H.ammodendron seedlings at early stage.NaCl stress had higher germination rate,germination potential,germination index,seedling vitality index,seedling length and root-to-head ratio than PEG stress.Conclusion:Soil drought and physiological drought significantly inhibited seed germination and seedling growth of H.ammodendron,but soil drought inhibition was stronger.H.ammodendron was more suitable for sowing seedlings in arid saline areas where the soil water potential not less than-2.25 Mpa.

关 键 词：梭梭 种子萌发 幼苗 PEG胁迫 NACL胁迫 

分 类 号：Q949[生物学—植物学]