Skin care efficacy study of recombinant humanized collagen based on in vitro level  

重组人源化胶原蛋白基于体外水平的护肤功效研究

作　　者：Jian Wang Yuhui Fan Danfeng Li Ningwen Cheng Ling Li Yufeng Yu 王建;范毓慧;李丹凤;程宁文;李玲;于玉凤(山西锦波生物医药股份有限公司功能蛋白山西省重点实验室,山西太原030032;山西医科大学,山西太原030012)

机构地区：[1]Shanxi Key Laboratory of Functional Proteins,Shanxi Jinbo Bio-Pharmaceutical Co.,Ltd.,Taiyuan,Shanxi 030032,China [2]Shanxi Medical University,Taiyuan,Shanxi 030012,China

出　　处：《日用化学工业（中英文）》2024年第9期1030-1038,共9页China Surfactant Detergent & Cosmetics

摘　　要：Studying the skin care efficacy of recombinant humanized collagen based on in vitro level.The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures,then its skincare efficacy based on in vitro level was evaluated by detecting the inhibition rate of elastase,the inhibition rate of collagenase,the protein content of type I collagen in human fibroblasts,the inhibition of reactive oxygen species(ROS)with human keratinocytes,and the effects of the recombinant humanized collagen on the expression of hyaluronic acid(HA),filaggrin(FLG)and transglutaminase 1(TGM1)in keratinocytes.The results showed that recombinant humanized collagen was able to maintain stability at temperatures below 70℃.With regard to its skincare efficacy,recombinant humanized collagen could inhibit elastase and collagenase activities and promote the increase of type I collagen content in human fibroblasts.It also showed good inhibition of ROS in keratinocytes in vitro and could increase the expression of HA,FLG,and TGM1 in keratinocytes.In short,the recombinant humanized collagen exhibited a favourable skin care effect in vitro level.This study proved that it has potential firming,anti-wrinkle,moisturizing,and repairing efficacy,and is a valuable cosmetic raw material.基于体外水平探究重组人源化胶原蛋白的护肤功效。首先通过不同温度对重组人源化胶原蛋白进行处理,分析其在不同温度条件下的稳定性,随后通过检测其对弹性蛋白酶的抑制率、胶原蛋白酶的抑制率、人成纤维细胞Ⅰ型胶原蛋白含量、体外角质形成细胞活性氧(ROS)抑制作用及对角质形成细胞透明质酸(HA)、丝聚蛋白(FLG)和转谷氨酰胺酶1(TGM1)表达量的影响,评价重组人源化胶原蛋白的体外护肤功效。结果显示,重组人源化胶原蛋白在70℃以内能保持其稳定性良好。在护肤功效方面,重组人源化胶原蛋白能够抑制弹性蛋白酶和胶原蛋白酶的活性,促进人成纤维细胞Ⅰ型胶原蛋白含量上升。同时,其对体外角质形成细胞ROS具有显著的抑制作用,并可以增加角质形成细胞HA、FLG和TGM1的表达量。总之,重组人源化胶原蛋白具有良好的体外护肤功效,本研究证明其具有潜在的紧致、抗皱、保湿及修护作用,是一种理想的化妆品原料。

关 键 词：recombinant humanized collagen stability human fibroblast cell in vitro keratinocytes skin care efficacy 

分 类 号：TQ658[化学工程—精细化工]