构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用及机制研究  

The protective effect and mechanism of crude polysaccharide from Broussonetia papyrifera flower on UV-induced skin photodamage

作　　者：赖梓漩 宋雨轩 段雪伟 刘诗芸 刘冰 张敏君 杨慧文 Zixuan Lai;Yuxuan Song;Xuewei Duan;Shiyun Liu;Bing Liu;Minjun Zhang;Huiwen Yang(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006,China;School of Nursing,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东药科大学护理学院,广东广州510006

出　　处：《日用化学工业（中英文）》2024年第9期1069-1077,共9页China Surfactant Detergent & Cosmetics

基　　金：国家级大学生创新创业训练计划项目(202310573012)。

摘　　要：研究构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用,并探讨其作用机制。采用雌性昆明鼠建立长波紫外线(UVA)诱导小鼠皮肤光损伤模型,并对小鼠背部皮肤进行评分。采用苏木素-伊红(HE)、Masson染色考察小鼠皮肤组织病理学变化、皮肤厚度和胶原纤维的变化;考察皮肤组织Hyp、抗氧化酶SOD、GSH-Px、CAT和MDA,TNF-α、IL-1β、IL-6的含量,MMPs、Nrf2、HO-1和NF-κB通路相关蛋白的表达。结果显示,构树花粗多糖凝胶可不同程度改善皮肤粗糙溃烂现象,抑制皮肤增厚,提高真皮层胶原含量,使胶原纤维排列趋于紧密;提高皮肤组织中SOD、GSH-Px和CAT活力,降低MDA含量(P<0.05);降低皮肤促炎因子TNF-α、IL-1β和IL-6含量(P<0.05);MMP-1和MMP-3含量明显降低(P<0.05),Nrf2蛋白和HO-1蛋白表达显著升高(P<0.01),p-p65/p65蛋白表达比例显著降低(P<0.05)。可见,构树花粗多糖对紫外线致小鼠皮肤光损伤具有显著的保护作用,其机制可能与构树花粗多糖激活Nrf2通路、增强SOD等抗氧化酶活性,抑制NF-κB通路有关。To investigate the protective effect of crude polysaccharide from Broussonetia papyrifera flower on skin photodamage,a total of 56 female Kunming mice were randomly divided into 7 groups:control group,model group,matrix gel group,vitamin A group,and crude polysaccharide low-dose,medium-dose and high-dose groups.The exposed back skin of the mice was irradiated by UVA to induce skin photodamage model,and the drugs were applied to the skin 1 hour before irradiation.After 8 weeks of continuous irradiation,the back skin was scored.The effects of crude polysaccharide from Broussonetia papyrifera flower on the back skin morphology,skin thickness and collagen fibers were investigated by hematoxylin-eosin and Masson staining.And the contents of skin hydroxyproline,SOD,GSH-Px,CAT,MDA,TNF-α,IL-1β,and IL-6,and the protein expressions of MMP-1/3,Nrf2,HO-1 and NF-κB pathway were also examined.Pre-treated skin with different dose of crude polysaccharide gel 1 hour before UVA irradiation can improve roughness and ulceration of the back skin to different degrees,ameliorate skin thickening,and dermal collagen damage.At the same time,the activities of SOD,GSH-Px and CAT in skin tissue are increased,and the content of MDA is decreased(P<0.05).The contents of pro-inflammatory cytokines,TNF-α,IL-1βand IL-6,are decreased(P<0.05).The contents of MMP-1 and MMP-3 are decreased significantly(P<0.05),the expression of Nrf2 and HO-1 protein are increased(P<0.01),and p-p65/p65 protein are decreased significantly(P<0.05)in the mouse back skin by pre-treatment with crude polysaccharide gel.The crude polysaccharide from Broussonetia papyrifera flower has a strong antioxidant activity in vitro,and a protective effect on UVAinduced mice skin photodamage in vivo.The mechanism may be related to the activation of Nrf2 and inhibition of NF-κB signaling pathway,leading to the regulation of antioxidative enzyme SOD and improvement of inflammatory response.

关 键 词：构树花粗多糖 皮肤光损伤 氧化应激 炎症因子 Nrf2通路 

分 类 号：TQ658[化学工程—精细化工]