白花泡桐叶片提取物的抗皮肤光老化作用研究  

Study on the anti-photoaging effect of Paulownia fortunei leaf extract

作　　者：孙琳 张曼[2] Lin Sun;Man Zhang(Henan Provincial Defense Science and Technology Intelligence Information and Engineering Quality Supervision Station(Henan Provincial Space Information Data and Application Center),Zhengzhou,Henan 450003,China;Henan Agricultural University,Zhengzhou,Henan 450002,China)

机构地区：[1]河南省国防科技情报信息与工程质量监督站(河南省空间信息数据与应用中心),河南郑州450003 [2]河南农业大学,河南郑州450002

出　　处：《日用化学工业（中英文）》2024年第9期1092-1098,共7页China Surfactant Detergent & Cosmetics

摘　　要：考察白花泡桐叶片提取物(Paulownia fortunei leaf extract,PFLE)对紫外线B(UVB)诱导的皮肤光老化小鼠的影响。将小鼠分为5组:分别为正常对照组(NC)、模型组(M)、低剂量PFLE组(L-PFLE)、中剂量PFLE组(M-PFLE)、高剂量PFLE组(H-PFLE)。NC组小鼠不照射。其他组小鼠使用紫外线光疗仪进行UVB照射。UVB照射当天,NC组和M组小鼠背部涂抹雪花膏,L-PFLE组、M-PFLE组和H-PFLE组小鼠分别涂抹50,100和200 mg/kg的PFLE乳膏。UVB照射和给药周期均为6周。检测了小鼠的体重、皮肤含水量、皮肤氧化应激指标、HYP和HA水平。采用HE染色观察小鼠皮肤形态。通过Western blotting检测了小鼠皮肤中Nrf2(细胞核)、Keap1和HO-1的蛋白表达水平。通过RT-qPCR检测了小鼠皮肤中MMP-1、MMP-3的转录水平。结果显示,与M组比较,L-PFLE组、M-PFLE组和H-PFLE组小鼠皮肤含水量升高,皮肤形态明显改善,SOD、CAT和GSH-Px水平升高,MDA水平降低,Nrf2(细胞核)和HO-1蛋白相对表达量升高,Keap1蛋白相对表达量降低,HYP和HA水平升高,MMP-1和MMP-3 mRNA相对表达量降低(P<0.05)。表明PELE经皮给药具有良好的抗皮肤光老化作用。The study aimed to investigate the effect of Paulownia fortunei leaf extract(PFLE)on ultraviolet B(UVB)-induced skin photoaging in mice.The mice were divided into 5 groups:normal control group(NC),model group(M),low-dose PFLE group(L-PFLE),medium-dose PFLE group(M-PFLE),and high-dose PFLE group(H-PFLE).Mice in NC group were not irradiated.Mice in the other groups were irradiated with UVB using ultraviolet photo-therapy equipment.On the day of UVB irradiation,mice in NC group and M group were smeared with cream on their backs,and mice in L-PFLE,M-PFLE and H-PFLE groups were smeared with 50,100 and 200 mg/kg PFLE cream,respectively.The UVB irradiation and administration cycles were both 6 weeks.The body weight,skin moisture content,skin oxidative stress indicators(SOD,CAT,GSH-Px and MDA),HYP and HA levels of the mice were detected.HE staining was used to observe skin morphology.The protein expression levels of Nrf2(nucleus),Keap1 and HO-1 in the skin were detected by Western blotting.The transcript levels of MMP-1 and MMP-3 in the skin were detected by RT-qPCR.The results show that compared with M group,the skin moisture content of the mice in L-PFLE group,M-PFLE group and H-PFLE group increases,the skin morphological changes are significantly improved,the levels of SOD,CAT and GSHPx increase,the MDA level decreases,the relative expressions of Nrf2(nucleus)and HO-1 protein increase,the relative expression of Keap1 protein decreases,the levels of HYP and HA increase,and the relative expressions of MMP-1 and MMP-3 mRNA decrease(P<0.05).This study shows that transdermal administration of PELE has a good anti-skin photoaging effect.

关 键 词：白花泡桐叶片提取物 皮肤光老化 抗氧化 羟脯氨酸 透明质酸 

分 类 号：TQ658[化学工程—精细化工]