丝素蛋白对皮肤光损伤的保护作用  

Protective effect of silk fibroin on UVB-induced skin injury

作　　者：李生鹏 李静 梁超 赵冉 张晓洁 孙丽丽 Shengpeng Li;Jing Li;Chao Liang;Ran Zhao;Xiaojie Zhang;Lili Sun(Beijing Eviskin Inc.,Beijing 102401,China)

机构地区：[1]三立慧评(北京)检测技术有限公司,北京102401

出　　处：《日用化学工业（中英文）》2024年第9期1117-1124,共8页China Surfactant Detergent & Cosmetics

摘　　要：丝素蛋白是一种从蚕丝中提取的天然高分子纤维蛋白,有很好的抗氧化和减少光损伤的作用。为了研究丝素蛋白对皮肤光损伤的保护作用,以UVB诱导人角质形成细胞(HaCaT)损伤为模型,通过ELISA和荧光检测细胞内活性氧(ROS)和钙离子(Ca^(2+))的水平,采用免疫荧光检测瞬时受体电位阳离子通道亚家族V成员1(TRPV1)和Claudin-1的表达。此外,还使用蛋白质印迹分析了紧密连接相关蛋白Occludin和Claudin-1的表达。结果表明,120 m J/cm^(2)的UVB刺激显著降低细胞活力,而100μg/m L丝素蛋白的存在显著增加细胞活力。UVB刺激导致细胞内活性氧水平升高,激活TRPV1通道,诱导细胞内Ca^(2+)水平增加。同时炎症因子白细胞介素1α(IL-1α)(P<0.01)和S100钙结合蛋白A8(S100A8)(P<0.05)水平也显著升高,引发炎症反应。添加丝素蛋白的HaCaT细胞受到UVB的影响则显著降低(P<0.05)。实验证明,UVB会破坏屏障蛋白,导致屏障蛋白Claudin-1和Occludin的表达下降。丝素蛋白的加入可以降低这种影响,保护细胞间紧密连接。因此,丝素蛋白可能具有降低氧化应激、屏障损伤和炎症发生,保护皮肤免受UVB诱导的光损伤的潜在能力。As a natural macromolecular protein extracted from silk,silk fibroin(SF)has been widely used as biological material in recent years due to its antioxidant and photodamage reduction effects.To study the protective effect of silk fibroin against skin photodamage,the immortalized human keratinocyte line(HaCaT)was used as a model for study of UVB injury.Intracellular reactive oxygen species(ROS)and the levels of calcium ions(Ca^(2)+)were measured by ELISA and fluorescence microscopy.The expression of transient receptor potential cation channel subfamily V member 1(TRPV1)and the expression of Claudin-1 were detected by immunofluorescence assay.In addition,western blotting was used to analyze the expression of tight junction proteins occludin and Claudin-1.The results showed that 120 mJ/cm^(2)of UVB stimulation significantly reduced cell viability,while the presence of 100μg/mL silk fibroin significantly increased cell viability.UVB stimulation could increase the level of intracellular ROS,activate TRPV1 channels,and induce the increase of intracellular Ca^(2+)level.Meanwhile,the levels of inflammatory cytokines interleukin-1α(IL-1α)(P<0.01)and S100A8(P<0.05)were also significantly increased,which caused inflammation.However,the effect of UVB on HaCaT cells with the addition of silk fibroin was significantly decreased(P<0.05).In conclusion,UVB could disrupt the structural integrity of barrier proteins,resulting in the decreased expression of the barrier proteins Claudin-1 and occludin.The addition of silk fibroin could reduce such effect and protect the tightly connected structure.Therefore,silk fibroin might have great potential to protect skin from UV-induced inflammation,barrier damage and oxidative stress damage.

关 键 词：丝素蛋白 UVB 活性氧 紧密连接蛋白 皮肤屏障 

分 类 号：TQ658[化学工程—精细化工]