进口高粱中3种检疫性病毒的检测  

Detection of three quarantinee viruses in imported sorghum

作　　者：陈红运 廖富荣 陈青 叶明辉 冯黎霞 于翠 王秀芬 Chen Hongyun;Liao Furong;Chen Qing;Ye Minghui;Feng Lixia;Yu Cui;Wang Xiufen(Xiamen Customs District Technology Center,Xiamen 361026,China;Guangzhou Customs District Technology Center;Shanghai Customs District Animal,Plant and Food Inspection and Quarantine Technology Center;Dalian Customs District Technology Center)

机构地区：[1]厦门海关技术中心,福建厦门361026 [2]广州海关技术中心 [3]上海海关动植物与食品检验检疫技术中心 [4]大连海关技术中心

出　　处：《植物检疫》2024年第5期26-30,共5页Plant Quarantine

基　　金：海关总署科研项目(2022HK009,2024HK040)。

摘　　要：本研究以进口高粱为材料,采用一步法RT-PCR检测玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)、玉米矮花叶病毒(maize dwarf mosaic virus,MDMV)和小麦线条花叶病毒(wheat streak mosaic virus,WSMV)。23份高粱样品中,2份检出MCMV,5份检出MDMV,19份样品检出WSMV。1份样品检出MCMV、MDMV和WSMV,4份样品检出MDMV和WSMV。综合前期和本研究结果,建议将MCMV-F7/MCMV-R7、MDMV482F/MDMV482R和WSMV-F3/WSMV-R3作为3种检疫性病毒的首选检测引物,MCMV-2452F/MCMV-3111R、3MDF/1MDR和WSMV-1F/WSMV-3R作为备选,引物3MDF/1MDR用于鉴定MDMV分离物的分支归属。This study used imported sorghum as material to detect maize chlorotic mottle virus(MCMV),maize dwarf mosaic virus(MDMV),and wheat stripe mosaic virus(WSMV)based on one-step RT-PCR.Among 23 sorghum samples,2 samples were positive for MCMV,5 samples were positive for MDMV,and 19 samples were positive for WSMV.One sample tested positive for MCMV,MDMV and WSMV,while 4 samples were positive for MDMV and WSMV.It is recommended to use MCMV-F7/MCMV-R7,MDMV482F/MDMV482R and WSMV-F3/WSMV-R3 as the preferred primers for the detection of three quarantine viruses.MCMV-2452F/MCMV-3111R,3MDF/1MDR and WSMV-1F/WSMV-3R are considered as candidates,and the primer 3MDF/1MDR is used to identify the phylogroup of MDMV isolates.

关 键 词：玉米褪绿斑驳病毒 玉米矮花叶病毒 小麦线条花叶病毒 检测 

分 类 号：S432.41[农业科学—植物病理学]