鳙IGFBP1分子特征及基因表达模式研究  

MOLECULAR CHARACTERIZATION AND EXPRESSION ANALYSES OF IGFBP1(INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1)GENE IN BIGHEAD CARP(HYPOPHTHALMICHTHYS NOBILIS)

作　　者：张正 俞小牧[2] 陈庚 邹钧 童金苟[2] ZHANG Zheng;YU Xiao-Mu;CHEN Geng;ZOU Jun;TONG Jin-Gou(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;State Key Laboratory of Freshwater Ecology and Biotechnology,Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture of Chinese Academy of Sciences,Institute of Hydrobiology of Chinese Academy of Sciences,Wuhan 430072,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国科学院水生生物研究所淡水生态学与生物技术国家重点实验室,中国科学院水产品种创制与高效养殖重点实验室,武汉430072

出　　处：《水生生物学报》2024年第11期1926-1933,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(32072973)资助。

摘　　要：从鳙(Hypophthalmichthys nobilis)基因组中获得胰岛素样生长因子结合蛋白1(IGFBP1,insulin-like growth factor binding protein 1)基因序列,并对其蛋白质理化和进化特征以及基因表达模式进行分析。鳙IGFBP1开放阅读框(ORF)为789 bp,编码262个氨基酸,蛋白分子量为28139.23 u,等电点(pI)为7.42,具有1个信号肽但不具有跨膜结构,推测其为亲水性分泌蛋白。鳙IGFBP1蛋白具有两个保守的IB和TY结构域,与鲤形目鱼类IGFBP1有较高的序列同源性。鳙IGFBP1的基因时空表达分析结果显示:体节期表达量显著上调,出膜后表达水平迅速下降;成鱼肝脏中的表达量最高,皮肤、肠、性腺等组织也有一定的表达,其余组织的表达量较低。对群体中具有显著生长差异的个体进行IGFBP1表达分析,结果显示在极大个体与极小个体的肝脏组织中具有极显著性差异(P<0.01),提示该基因在鳙快速生长中发挥了正调控作用。研究结果对鳙生长调控分子机制研究及遗传改良研究具体理论和潜在应用价值。The gene sequence of insulin-like growth factor binding proteins 1(IGFBP1)was obtained from the genome of bighead carp(Hypophthalmichthys nobilis),and subsequently,the physicochemical and evolutionary characteristics of the IGFBP1 protein and gene expression were analyzed.The open reading frame(ORF)of bighead carp IGFBP1 is 789 bp,encoding 262 amino acids.The protein has a molecular weight of 28139.23 u and an isoelectric point(pI)of 7.42.Notably,it possesses a signal peptide but lacks a transmembrane structure,suggesting its classification as a hydrophilic secreted protein.Additionally,the IGFBP1 protein in bighead carp contains two conserved IB and TY domains,demonstrating high sequence homology with IGFBP1 in cypriniformes.Spatial and temporal expression analyses of the IGFBP1 gene in bighead carp revealed significantly up-regulation during the segmental stage,contrasting with lower expression levels during the hatching period.In adult fish,the highest expression level was observed in liver,followed by skin,intestine,and gonad,while expression in other tissues remained relatively low.Differential growth analysis showed significant differences in IGFBP1 in liver tissues between large(heavy group)and small(light group)fish(P<0.01),suggesting a positive regulatory role for the IGFBP1 gene in facilitating the rapid growth of bighead carp.The results of this study hold both theoretical significance and potential applications in further elucidating the molecular mechanisms underlying growth regulation and in implementing genetic improvements for farmed bighead carp.

关 键 词：IGFBP1 分子特征 基因表达 鳙 

分 类 号：Q344.1[生物学—遗传学]