Ag^(+)/Cys介导的核酸探针级联荧光信号免疫检测法测定玉米和大米中黄曲霉毒素B_(1)  

A Method for Determination of Aflatoxin B_(1)in Corn and Rice by Using Ag^(+)/Cys Mediated Nucleic Acid Probe Cascade Immunofluorescence Assay

作　　者：李亚楠 解立斌[1,2] 陈佳 韩爱云 张文娜[1,2] 剧慧栋 冯佩[3] 张媛媛 胡连霞 Li Yanan;Xie Libin;Chen Jia;Han Aiyun;Zhang Wenna;Ju Huidong;Feng Pei;Zhang Yuanyuan;Hu Lianxia(Chemical Engineering Institute,Shijiazhuang University,Shijiazhuang 050035;Shijiazhuang Food Engineering Technology Innovation Center,Shijiazhuang 050035;Physical and Chemical Laboratory,Shijiazhuang Center for Disease Prevention and Control,Shijiazhuang 050011)

机构地区：[1]石家庄学院化工学院,石家庄050035 [2]石家庄市食品工程技术创新中心,石家庄050035 [3]石家庄市疾病预防控制中心理化检验科,石家庄050011

出　　处：《中国粮油学报》2024年第8期74-81,共8页Journal of the Chinese Cereals and Oils Association

基　　金：石家庄市科学技术研究与发展计划项目(211170641);石家庄学院博士科研启动基金项目(21BS002);石家庄学院校级教学改革研究与实践项目(JGXM-202170G);河北省高层次人才资助项目(B20221010)。

摘　　要：以葡萄糖氧化酶(GOx)催化Ag^(+)/Cys介导的富G-ssDNA/Tb^(3+)核酸探针体系引发荧光强度变化为方法学基础,开发了一种新型ELISA双重级联荧光信号放大系统,对葡萄糖氧化酶具有高的灵敏度响应(低至0.05μg/mLGOx)。将此体系与对GOx负载的纳米微球为信号探针的磁分离免疫体系相结合,建立了一种用于食品中AFB_(1)的高灵敏的双重级联信号放大的荧光免疫检测方法。该方法对AFB_(1)的检出限为0.05 ng/mL,在加标的玉米和大米样品中,添加回收实验结果表明,AFB_(1)的回收率在85.09%~105.2%之间,变异系数(CV)值为6.8%~15.7%。Based on the fluorescence intensity changes of Glucose Oxidase(GOx)catalyzed Ag^(+)/Cys mediated G-rich ssDNA/Tb^(3+)nucleic acid probe,a novel double cascade fluorescence signal amplification system was developed.The fluorescence system had a high sensitivity response to Gox(as low as 0.05μg/mlGOx).This system was combined with magnetic separation immune system to establish a high-sensitivity double cascade signal amplification fluorescence immunoassay method for detection AFB_(1)in foods.The detection limit of this method for AFB_(1)was 0.05 ng/mL.The results of the addition recovery experiment indicated that the recovery rate of AFB_(1)was 85.09%~105.2%,with the coefficient of variation(CV)was 6.8%~15.7%.

关 键 词：黄曲霉毒素B_(1)(AFB_(1)) 富G单链DNA 铽离子(Tb^(3+)) 抗体 荧光免疫检测 

分 类 号：O657.39[理学—分析化学]