自动化磁珠法前处理在液相色谱串联质谱检测血浆儿茶酚胺及其代谢物中的应用  

The Application of Automated Magnetic Bead Pretreatment in the Detection of Catecholamines and Their Metabolites in Plasma by Liquid Chromatography Tandem Mass Spectrometry

作　　者：李艳晓 Li Yanxiao(Tianjin Fourth Central Hospital,Tianjin 300100,China)

机构地区：[1]天津市第四中心医院,天津300100

出　　处：《医疗装备》2024年第17期19-24,共6页Medical Equipment

摘　　要：目的探讨自动化磁珠法前处理在液相色谱串联质谱(LC-MS/MS)检测血浆儿茶酚胺及其代谢物中的应用效果。方法采用自动化磁珠法提取血浆标本中的儿茶酚胺[去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA)]及其代谢物[甲氧基去甲肾上腺素(NMN)、甲氧基肾上腺素(MN)、3-甲氧酪胺(3-MT)],并采用LC-MS/MS检测,分析该检测方法的线性、准确度、精密度及基质效应(ME)等性能。结果NE、E、DA、NMN、MN、3-MT检测的线性相关系数r2均>0.99,各浓度值与标准值偏差范围为0%~14.84%,均<15%。NE、E、DA、NMN、MN、3-MT的低、中、高值质控品批内变异系数(CV)为0%~13.480%,批间CV为1.260%~13.590%,符合批内、批间CV均≤15%的要求。NE、E、DA、NMN、MN、3-MT的ME均在-20%~20%范围内,说明ME影响较小。结论LC-MS/MS检测儿茶酚胺及其代谢物采用自动化磁珠法进行血浆标本前处理,各指标检测的线性、准确度、精密度及ME均符合行业标准。Objective To investigate the effectiveness of automatic magnetic bead pretreatment in the detecting catecholamines and their metabolites in plasma by liquid chromatography tandem mass spectrometry(LC-MS/MS).Methods With the use of Automated magnetic bead method to extract catecholamines[norepinephrine(NE),epinephrine(E),dopamine(DA)]and their metabolites[methoxynorepinephrine(NMN),methoxyepinephrine(MN),3-methoxytyramine(3-MT)]from plasma samples and the use of LC-MS/MS to detect,the linearity,accuracy,precision,and matrix effects(ME)of this detection method were analyzed.Results The linear correlation coefficients r2 of NE,E,DA,NMN,MN and 3-MT detection were greater than 0.99,and the deviation range of each concentration value from the standard value was 0%~14.84%,all of which were less than 15%.The intra-batch coefficient of variation(CV)of low,medium and high values of NE,E,DA,NMN,MN and 3-MT was 0%~13.480%,and the inter-batch CV was 1.260%~13.590%,which met the requirements of both intra-batch and inter-batch CV≤15%.The ME of NE,E,DA,NMN,MN,and 3-MT were all in the range of-20%to 20%,indicating that the effect of ME was small.Conclusion With the use of LC-MS/MS for detecting catecholamines and their metabolites,and the use of automated magnetic bead method for plasma sample pretreatment,the linearity,accuracy,precision,and ME detection results for each indicator met industry standards.

关 键 词：自动化磁珠法 液相色谱串联质谱 儿茶酚胺 前处理 代谢物 性能验证 

分 类 号：R446[医药卫生—诊断学]