基于UHPLC-QTOF-MS的薜荔水提物的化学成分分析及Beas-2b细胞抗衰老活性  

Chemical composition analysis and anti-cellular senescence ability of aqueous extracts of Ficus pumila L.based on UHPLC-QTOF-MS

作　　者：莫世儒 叶小梅 王诚远[1] 王刚[1] 谢建华[1] MO Shiru;YE Xiaomei;WANG Chengyuan;WANG Gang;XIE Jianhua(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,江西南昌330047

出　　处：《南昌大学学报（工科版）》2024年第3期307-315,394,共10页Journal of Nanchang University(Engineering & Technology)

基　　金：国家重点研发计划资助项目(2023YFF1104001)。

摘　　要：采用超高效液相色谱-四级杆飞行时间-质谱(UHPLC-QTOF-MS)技术对薜荔果、茎、叶中化学成分进行分析,并对其缓解由香烟烟雾提取物诱导的肺部细胞衰老能力进行研究。以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,流速为0.2μL·min^(-1);采用电喷雾电离原(ESI),正负离子扫描模式进行数据采集,通过Msdial进行数据库比对和参考已报道文献对薜荔果、茎和叶3种部位的水提物进行化学成分表征。建立香烟烟雾提取物诱导人支气管上皮细胞Beas-2b衰老模型,测定薜荔果、茎、叶3种水提物对细胞活性及细胞衰老的影响。结果成功鉴定出薜荔果、茎、叶水提物中的33种化学成分,发现薜荔3种水提物在适宜质量浓度范围内对Beas-2b细胞无明显毒性,50μg·mL^(-1)的薜荔茎和叶水提物对细胞衰老有一定缓解作用。结果表明UHPLC-QTOF-MS法灵敏度高,分析快速,结果可靠,能够全面快速分析和鉴定薜荔不同部位水提物中的化学成分,薜荔具有潜在的抗细胞衰老能力。The chemical constituents in Ficus pumila L.fruits,stems and leaves were characterized by ultra-high performance liquid chromatography-quadrupole time-of-flight-mass spectrometry(UHPLC-QTOF-MS),and an investigation was conducted on their ability to alleviate lung cellular senescence induced by cigarette smoke extract.Acetonitrile-0.1%formic acid aqueous solution was used as the mobile phase for gradient elution at a flow rate of 0.2μL·min^(-1);electrospray ionization(ESI)was used with positive and negative ion scanning modes for data acquisition,and the aqueous extracts of Ficus pumila L.fruits,stems,and leaves were characterized by database comparison through Msdial and by referring to the reported literature.A Beas-2b senescence model of human bronchial epithelial cells induced by cigarette smoke extract(CSE)was used to investigate the effects of aqueous extracts of Ficus pumila L.fruits,stems,and leaves on cellular activities and senescence.The findings demonstrated that thirty-three chemical components in the aqueous extracts of Ficus pumila L.fruit,stem and leaf were characterized by combining database and literature reports.The three aqueous extracts of Ficus pumila L.showed no significant toxicity to Beas-2b cells in the appropriate concentration range.50μg·mL^(-1) of the aqueous extract of Ficus pumila stems and leaves showed some alleviation of cellular senescence.The results indicated that the UHPLC-QTOF-MS method is sensitive,rapid and reliable,and can comprehensively and rapidly analyze and characterize the chemical components in the aqueous extracts of different parts of Ficus pumila L.,and Ficus pumila L.has potential anti-cellular senescence ability.

关 键 词：薜荔 超高效液相色谱-四级杆飞行时间-质谱 化学组分 细胞衰老 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]