盐酸伊伐布雷定对阿霉素诱导的心力衰竭大鼠心室重构的影响  

Effect of ivabradine hydrochloride on ventricular remodeling in rats with adriamycin-induced heart failure

作　　者：王方泽 王韵菡 赵云波[1] 张瑞 王艳丽 马长虹 王健[1] WANG Fangze;WANG Yunhan;ZHAO Yunbo;ZHANG Rui;WANG Yanli;MA Changhong;WANG Jian(Department of Cardiology,Weifang People’s Hospital,Shandong,Weifang 261000,China;School of Public Health,Shandong Second Medical University,Shandong,Weifang 261000,China;Department of Nursing,Weifang NO.2 People’s Hospital,Shandong,Weifang 261000,China)

机构地区：[1]山东省潍坊市人民医院心血管内科,山东潍坊261000 [2]山东第二医科大学公共卫生学院,山东潍坊261000 [3]山东省潍坊市第二人民医院护理部,山东潍坊261000

出　　处：《中国医药科学》2024年第18期4-9,共6页China Medicine And Pharmacy

基　　金：山东省自然科学基金青年项目(ZR2021QH262);山东省潍坊市科技发展计划项目(2020YX010)。

摘　　要：目的探讨盐酸伊伐布雷定对阿霉素诱导的心力衰竭大鼠心室重构的影响。方法50只无特定病原体级9周龄Wistar大鼠[体重(300±10)g,雌雄不限],按照随机数表法分为对照组(10只)、模型组(20只)、治疗组(20只)。采用阿霉素诱导法构建心力衰竭模型,以左室射血分数(LVEF)35%~50%为建模成功的标准。治疗组盐酸伊伐布雷定[10 mg/(kg·d)]灌胃,对照组和模型组等量生理盐水灌胃,4周后,心脏超声记录左室收缩末期内径(LVEDs)、左室舒张末期内径(LVEDd)、LVEF、左室短轴缩短率(LVFS)变化,苏木精-伊红染色观察左室心肌细胞,Luminex、蛋白免疫印迹分析、酶联免疫吸附试验检测单核细胞趋化蛋白1(MCP-1)、肿瘤坏死因子-α(TNF-α)、脑钠肽(BNP)、白细胞介素-1β(IL-1β)、粒细胞集落刺激因子(G-CSF)等因子水平。结果与对照组比较,模型组LVEDs、LVEDd增加(P<0.05),LVEF、LVFS降低(P<0.05);MCP-1、TNF-α、IL-1β、G-CSF、BNP等因子水平升高(P<0.05)。与模型组比较,治疗组LVEDs、LVEDd降低(P<0.05),LVEF、LVFS增加(P<0.05),且上述因子表达水平降低(P<0.05)。对照组心肌细胞形态正常,模型组心肌细胞明显异常并伴有炎症细胞浸润,治疗后得到部分缓解。结论腹腔注射阿霉素的方法制备大鼠心力衰竭模型成功率高且稳定,盐酸伊伐布雷定可以有效抑制该模型的炎症反应,改善心室重构。Objective To investigate the effect of ivabradine on ventricular remodeling in rats with adriamycin-induced heart failure.Methods 50 specific pathogen-free grade 9-week-old Wistar rats(weighing about[300±10]g,male and female)were divided into control group(10 rats),model group(20 rats),and treatment group(20 rats)using a randomized numerical table method.Adriamycin-induced method was used to construct a rat heart failure model,and left ventricular ejection fraction(LVEF)35%-50%was taken as the criterion for model completion.Ivabradine hydrochloride(10 mg/[kg·d])was given in the treatment group,and an equal amount of saline was given in the control and model group.After 4 weeks,changes in left ventricular end-systolic diameter(LVEDs),left ventricular end-diastolic diameter(LVEDd),LVEF,and left ventricular fractional shortening(LVFS)were recorded by cardiac ultrasound.Hematoxylin-eosin staining was performed to observe the left ventricular cardiomyocytes,and Luminex,protein immunoblotting,and enzyme-linked immunosorbent assay were used to detect the levels of monocyte chemotactic protein 1(MCP-1),tumor necros is factorα(TNF-α),brain natriuretic peptide(BNP),interleukin 1β(IL-1β),granulocyte colony-stimulating factor(G-CSF),and other factors.Results Compared with the control group,LVEDs and LVEDd were increased(P<0.05)and LVEF and LVFS were decreased in the model group(P<0.05),and the expression levels of factors such as MCP-1,TNF-α,IL-1β,G-CSF and BNP were increased(P<0.05).Compared with the model group,LVEDs and LVEDd were reduced(P<0.05)and LVEF and LVFS were increased in the treatment group(P<0.05).and the levels of the above factors were reduced(P<0.05).The morphology of cardiomyocytes in the control group was normal,and cardiomyocytes in the model group were obviously abnormal with inflammatory cell infiltration,which was partially relieved after treatment.Conclusion The method of intraperitoneal injection of amiodarone has a high and stable success rate in preparing a rat heart failure model.Ivabradin

关 键 词：心力衰竭 盐酸伊伐布雷定 心室重构 炎症 

分 类 号：R541.6[医药卫生—心血管疾病]