Morphological,phylogenetic and metabolite profile of Prorocentrum clipeus,a newly recorded epiphytic dinoflagellate in the northern Yellow Sea  

作　　者：Ruifang Wang Mengmeng Tong Shiwen Zhou Junjie Zheng Wenguang Zhang Xinfeng Dai Douding Lu Jiarong Hu Tianze Leng Qinglin Mu Zhongyong Yan Jiangning Zeng Pengbin Wang 

机构地区：[1]Ocean College,Zhejiang University,Zhoushan 316021,China [2]Key Laboratory of Marine Ecosystem Dynamics,Second Institute of Oceanography,Ministry of Natural Resources,Hangzhou 310012,China [3]The State Key Laboratory of Marine Pollution,Department of Biomedical Sciences,City University of Hong Kong,Hong Kong SAR 999077,China [4]Zhejiang Marine Ecology and Environment Monitoring Center,Zhoushan 316000,China

出　　处：《Acta Oceanologica Sinica》2024年第8期128-141,共14页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract Nos 41706191 and 41961144013;the Natural Science Foundation of Zhejiang Province under contract No.LY20D060004;the National Natural Science Foundation of China under contract Nos 41676111,41876139 and 41906140;the Program of Bureau of Science and Technology of Zhoushan Grant under contract No.2019C81031;the Basic Public Welfare Research Project of Zhejiang Province under contract No.LGC22B050032.

摘　　要：More than 30 species of benthic Prorocentrum have been identified,some of which produce okadaic acid(OA)and its derivatives,dinophysistoxins(DTXs),which cause diarrhetic shellfish poisoning(DSP).Increasing numbers of benthic Prorocentrum species have been reported in tropical and subtropical waters of China.In contrast,only a few benthic Prorocentrum species have been reported in temperate waters.In this study,morphological descriptions obtained using light microscopy,scanning electron microscopy and molecular characterization of one Prorocentrum clipeus strain isolated from the Yellow Sea are presented.Prorocentrum clipeus cells were nearly circular in shape,with a collar,ridge,and one protrusion.The periflagellar area was wide U-shaped,with two curved projections on platelet 1a.Nine periflagellar platelets of different sizes were observed.The morphology closely fits that of the species isolated from other locations.Phylogenetic analysis based on the molecular sequences of the small subunit(SSU)rDNA,internal transcribed spacer(ITS),and large subunit(LSU)rDNA was performed.A comprehensive metabolomic analysis incorporating target,suspect and non-target screenings was first applied to investigate the intracellular and extracellular metabolite profiles of the current isolate of P.clipeus.According to the results of the target and suspect screenings,179 metabolites or toxins produced by DSP-related algal species,including OA,dinophysistoxin-1(DTX1),dinophysistoxin-2(DTX2)and pectenotoxin-2(PTX2),were not detected.Non-target screening involving feature-based molecular networking(FBMN)provided a global view of major metabolites produced by the P.clipeus DF128 strain and revealed 23 clusters belonging to at least 13 compound classes,with organometallic compounds,lipids and lipid-like molecules,phenylpropanoids and polyketides,and benzenoids as major types.To date,this is the first record of the characterization of P.clipeus in samples from Chinese waters.Our results support the wide distribution of epibenthic Prorocent

关 键 词：PROROCENTRUM taxonomy PHYLOGENY metabolite profiling Yellow Sea 

分 类 号：Q949.2[生物学—植物学]