Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars  

作　　者：Yinghui Wu Yamei Li Yueyue Miao Hongkui Wei Hefeng Luo Chunxiao Ren Yawei Zhang Juan Chen Tanghong Wei Jiyan Deng Jian Peng 

机构地区：[1]Department of Animal Nutrition and Feed Science,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 400700,China [3]Frontiers Science Center for Animal Breeding and Sustainable Production,Wuhan 430070,China [4]Dekon Food and Agriculture Group,Chengdu 610000,China [5]Xingjia Bio-Engineering Co.Ltd,Changsha 410011,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第5期2044-2058,共15页畜牧与生物技术杂志（英文版）

基　　金：China Agriculture Research System(CARS-36);Major Project of Technical Innovation in Hubei Province(No.2022BBA0056);Dekon-Huazhong Agricultural University Project Co-operation Agreement;School-Enterprise Cooperation Project-Micronutrient Nutrition and Mechanism of Breeding Pigs。

摘　　要：Background Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia,which suggested that the current level and form of iron supplementation in boar diets may be inappropriate.Therefore,56 healthy Topeka E line boars aged 15–21 months were randomly divided into 5 groups:basal diet supplemented with 96 mg/kg ferrous sulfate(FeSO_(4))and 54 mg/kg glycine chelated iron(Gly-Fe,control);80 mg/kg or 115 mg/kg Gly-Fe;80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron(MHA-Fe,from CalimetFe)for 16 weeks.The effects of dietary iron supplementation with different sources and levels on semen quality in boars were investigated.Results 1)Serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group(P>0.05).2)Serum interleukin-6(IL-6)and sperm malondialdehyde(MDA)levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group(P<0.05),and higher serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group(P<0.05).3)Boars in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin(P<0.01),ferritin(P<0.05),and transferrin receptor(P<0.01)concentrations,and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group.4)Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the trial.However,the effect of Gly-Fe on improving semen quality in boars was not evident.5)Serum IL-6 level was positively correlated with hepcidin concentration(P<0.05),which in turn was significantly positively correlated with abnormal sperm rate(P<0.05).Furthermore,significant correlations were also found between indicators of iron status and oxidative stress and semen quality parameters.Conclusions Dietary supplementation with 80 mg/kg or 115

关 键 词：Adult boars Iron level Iron source Iron status Semen quality 

分 类 号：S828.5[农业科学—畜牧学]