Postbiotics from Saccharomyces cerevisiae fermentation stabilize microbiota in rumen liquid digesta during grain-based subacute ruminal acidosis(SARA) in lactating dairy cows  

作　　者：Junfei Guo Zhengxiao Zhang Le Luo Guan Ilkyu Yoon Jan C.Plaizier Ehsan Khafipour 

机构地区：[1]Department of Animal Science,University of Manitoba,Winnipeg,MB R3T 2N2,Canada [2]Present Address:College of Food and Biological Engineering,Jimei University,Xiamen,Fujian 361021,China [3]Department of Agriculture,Food and Nutrition Department,University of Alberta,Edmonton,AB T6G 2R3,Canada [4]Faculty of Land and Food Systems,University of British Columbia,Vancouver,BC V6T 1Z4,Canada [5]Diamond V,Cedar Rapids,IA 52404,United States [6]Present Address:Cargill Animal Nutrition,15407 McGinty Road West,Wayzata,MN 55391,USA

出　　处：《Journal of Animal Science and Biotechnology》2024年第5期2059-2080,共22页畜牧与生物技术杂志（英文版）

基　　金：supported by grants from Natural Science and Engineering Research Council(NSERC)of Canada Collaborative Research and Development(CRD)program;NSERC Discovery program,Dairy Farmers of Manitoba,and Diamond V,USA,to EK and JCP。

摘　　要：Background Subacute ruminal acidosis(SARA)is a common metabolic disorder of high yielding dairy cows,and it is associated with dysbiosis of the rumen and gut microbiome and host inflammation.This study evaluated the impact of two postbiotics from Saccharomyces cerevisiae fermentation products(SCFP)on rumen liquid associated microbiota of lactating dairy cows subjected to repeated grain-based SARA challenges.A total of 32 rumen cannulated cows were randomly assigned to 4 treatments from 4 weeks before until 12 weeks after parturition.Treatment groups included a Control diet or diets supplemented with postbiotics(SCFPa,14 g/d Original XPC;SCFPb-1X,19 g/d Nutri Tek;SCFPb-2X,38 g/d Nutri Tek,Diamond V,Cedar Rapids,IA,USA).Grain-based SARA challenges were conducted during week 5(SARA1)and week 8(SARA2)after parturition by replacing 20%DM of the base total mixed ration(TMR)with pellets containing 50%ground barley and 50%ground wheat.Total DNA from rumen liquid samples was subjected to V3–V416S r RNA gene amplicon sequencing.Characteristics of rumen microbiota were compared among treatments and SARA stages.Results Both SARA challenges reduced the diversity and richness of rumen liquid microbiota,altered the overall composition(β-diversity),and its predicted functionality including carbohydrates and amino acids metabolic pathways.The SARA challenges also reduced the number of significant associations among different taxa,number of hub taxa and their composition in the microbial co-occurrence networks.Supplementation with SCFP postbiotics,in particular SCFPb-2X,enhanced the robustness of the rumen microbiota.The SCFP supplemented cows had less fluctuation in relative abundances of community members when exposed to SARA challenges.The SCFP supplementation promoted the populations of lactate utilizing and fibrolytic bacteria,including members of Ruminococcaceae and Lachnospiraceae,and also increased the numbers of hub taxa during non-SARA and SARA stages.Supplementation with SCFPb-2X prevented the fluctuations in the abu

关 键 词：Postbiotics Rumen microbiota Saccharomyces cerevisiae fermentation products SARA 

分 类 号：S823.5[农业科学—畜牧学]