Effect of atractylenolide Ⅲ on zearalenone-induced Snail1-mediated epithelial–mesenchymal transition in porcine intestinal epithelium  

作　　者：Na Yeon Kim Myoung Ok Kim Sangsu Shin Woo‑Sung Kwon Bomi Kim Joon Yeop Lee Sang In Lee 

机构地区：[1]Department of Animal Science and Biotechnology,Kyungpook National University,Sangju,Gyeong‑sangbuk‑do,37224,Republic of Korea [2]Research Institute for Innovative Animal Science,Kyungpook National University,Sangju,Gyeongsangbuk‑do 37224,Republic of Korea [3]National Institute for Korean Medicine Development,Gyeongsan 38540,Republic of Korea

出　　处：《Journal of Animal Science and Biotechnology》2024年第5期2081-2092,共12页畜牧与生物技术杂志（英文版）

基　　金：supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2022R1I1A3070740)。

摘　　要：Background The intestinal epithelium performs essential physiological functions,such as nutrient absorption,and acts as a barrier to prevent the entry of harmful substances.Mycotoxins are prevalent contaminants found in ani-mal feed that exert harmful effects on the health of livestock.Zearalenone(ZEA)is produced by the Fusarium genus and induces gastrointestinal dysfunction and disrupts the health and immune system of animals.Here,we evaluated the molecular mechanisms that regulate the effects of ZEA on the porcine intestinal epithelium.Results Treatment of IPEC-J2 cells with ZEA decreased the expression of E-cadherin and increased the expression of Snai1 and Vimentin,which induced Snail1-mediated epithelial-to-mesenchymal transition(EMT).In addition,ZEA induces Snail-mediated EMT through the activation of TGF-βsignaling.The treatment of IPEC-J2 cells with atractyle-nolideⅢ,which were exposed to ZEA,alleviated EMT.Conclusions Our findings provide insights into the molecular mechanisms of ZEA toxicity in porcine intestinal epi-thelial cells and ways to mitigate it.

关 键 词：Atractylenolide III Epithelial–mesenchymal transition IPEC-J2 cells SNAIL TGF-beta signaling ZEARALENONE 

分 类 号：S828.5[农业科学—畜牧学]