黄芪甲苷对多囊卵巢综合征小鼠性激素水平及卵巢衰老的影响  

Effects of astragaloside on sex hormone levels and ovarian senescence in mice with polycystic ovary syndrome

作　　者：李雅清 王兴旺 刘英 姚显盈 杨勇 王海龙[3] 高艳萍 LI Yaqing;WANG Xingwang;LIU Ying;YAO Xianying;YANG Yong;WANG Hailong;GAO Yanping(Clinical Research Laboratory,First People′s Hospital of Datong,Cultivation Base of Key Laboratory on Hormonal Metabolic Diseases in Perimenopausal Period Built by Health Commission of Shanxi Province and Datong City,Datong 037009,China;First Clinical College,Shanxi University of Traditional Chinese Medicine;School of Basic Medicine,Shanxi Medical University)

机构地区：[1]大同市第一人民医院临床科研实验室,围绝经期激素代谢性疾病厅市共建山西省重点实验室培育基地,大同037009 [2]山西中医药大学第一临床学院 [3]山西医科大学基础医学院

出　　处：《山西医科大学学报》2024年第8期1017-1023,共7页Journal of Shanxi Medical University

基　　金：大同市应用基础研究计划项目(2022061);大同市平台(基地)计划项目(2022085)。

摘　　要：目的探讨黄芪甲苷(astragaloside,AST)对多囊卵巢综合征(PCOS)小鼠性激素水平及卵巢衰老的影响。方法30只雌性C57BL/6小鼠随机分为:正常对照组、模型组、AST组,每组10只。小鼠连续21 d颈背部皮下注射脱氢表雄酮(70 mg/kg)联合腹腔注射D-半乳糖(200 mg/kg)制备PCOS模型;黄芪甲苷干预组在造模成功后每天1次黄芪甲苷(50 mg/kg)灌胃给药,连续4周。给药最后10 d通过阴道涂片观察各组小鼠动情周期;给药结束后计算卵巢指数,HE染色观察卵巢组织病理学变化,酶联免疫吸附试验(ELISA)检测血清卵泡刺激素(follicle-stimulating hormone,FSH)、雌二醇(estradiol,E2)、睾酮(testosterone,T)和促黄体生成素(luteinizing hormone,LH)水平。免疫组织化学法检测各组小鼠卵巢组织衰老标志物β半乳糖苷酶1(GLB1)、P16、磷酸化P65(p-P65)表达水平,免疫印迹法检测各组小鼠卵巢组织GLB1、P16、p-P65及P65蛋白表达水平。结果与对照组比较,模型组小鼠动情周期紊乱,卵巢指数增加(P<0.05),卵巢呈多囊样改变,血清中T和LH含量显著增加(P<0.05),E2和FSH含量显著降低(P<0.05),卵巢组织GLB1、P16、p-P65蛋白水平显著上调(P<0.05)。与模型组比较,AST组小鼠动情周期恢复,卵巢指数降低(P<0.05),卵巢多囊样明显减少,血清中T和LH含量显著减少(P<0.05),E2和FSH含量显著增加(P<0.05),卵巢组织中GLB1、P16和p-P65蛋白水平显著降低(P<0.05),P65表达水平未见明显变化。结论黄芪甲苷能有效平衡PCOS小鼠的性激素水平,缓解卵巢组织衰老,其机制可能与抑制NF-κB信号通路有关。Objective To investigate the effects of astragaloside on the levels of sex hormone and senescence of ovary tissues in mice with polycystic ovary syndrome(PCOS).Methods Thirty female C57BL/6 mice were randomly divided into normal control group,model group and astragaloside group,with 10 mice in each group.The PCOS model was induced by subcutaneous injection of dehydroepiandrosterone(DHEA,70 mg/kg)on the nape back combined with intraperitoneal injection of D-galactose(200 mg/kg)once daily for 21 consecutive days.The mice in AST group were orally administered with AST(50 mg/kg)once a day for 4 consecutive weeks after the PCOS mice were sucessfully established.During the last 10 days of administration,the vaginal smear was performed to assess the estrus cycle.After the administration,the ovarian index was calculated.HE staining was conducted to observe the histopathological changes of the ovary.Serum levels of sex hormones testosterone(T),estradiol(E2),luteinizing hormone(LH)and follicle-stimulating hormone(FSH)were measured by ELISA.The protein levels of GLB1,P16,and p-P65 in ovarian tissues were detected by immunohistochemistry.The protein levels of GLB1,P16,p-P65 and P65 in ovarian tissues were detected by Western blot.Results Compared with control group,the oestrous cycle of mice was disorder in model group,the ovarian index was elevated(P<0.05),and the ovary was polycystic;the serum levels of T and LH were significantly enhanced(P<0.05),while the contents of E2 and FSH were significantly declined(P<0.05);and the levels of GLB1,P16 and p-P65 were augmented(P<0.05).Compared with PCOS group,the oestrous cycle of mice was restored in AST group,and the ovarian index was decreased(P<0.05);the serum levels of T and LH were significantly decreased(P<0.05),while the contents of E2 and FSH were significantly increased(P<0.05);and the levels of GLB1,P16,and p-P65 were reduced(P<0.05).The level of P65 showed no significant difference between groups.Conclusion AST can effectively balance the levels of sex hormone in PCOS mic

关 键 词：黄芪甲苷 多囊卵巢综合征 C57BL/6小鼠 性激素 细胞衰老 NF-κB信号通路 

分 类 号：R711[医药卫生—妇产科学]