大麦不同抗旱品种抗氧化酶活性及其相关基因表达分析  

Analysis of Antioxidant Enzyme Activity and Related Gene Expression in Different Drought-Resistant Barley Varieties

作　　者：郭姜维 姚立蓉 司二静[1,2] 汪军成[1,2] 马小乐[1,2] 李葆春[1,3] 王化俊[1,2] 郭传虎 万广有 孟亚雄[1,2] GUO Jiangwei;YAO Lirong;SI Erjing;WANG Juncheng;MA Xiaole;LI Baochun;WANG huajun;GUO Chuanhu;WAN Guangyou;MENG Yaxiong(State key Laboratory of Aridland Grop Science/State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province,Lanzhou,Gansu 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China)

机构地区：[1]省部共建干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《麦类作物学报》2024年第10期1262-1269,共8页Journal of Triticeae Crops

基　　金：国家大麦青稞产业技术体系项目(CARS-05-03B-03);国家自然科学基金项目(31960426)。

摘　　要：干旱是影响大麦生产的主要环境胁迫因子。为了解干旱胁迫下大麦抗氧化酶活性及相关基因表达变化规律,本试验以大麦抗旱品种ZDM5430和干旱敏感品种IL-12为材料,在大麦苗期进行干旱胁迫21 d后复水7 d处理,研究不同大麦品种在干旱胁迫及复水后叶片中抗氧化酶活性及基因表达的差异。结果表明,与对照相比,干旱胁迫下两个大麦品种的叶片相对含水量(RWC)和叶绿素(Chl)含量显著下降,丙二醛(MDA)、超氧阴离子自由基( O_(2)^(-·))和过氧化氢(H_(2)O_(2))含量显著升高,超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)活性显著增加。与抗旱品种ZDM5430相比,干旱胁迫下敏感品种IL-12的RWC和Chl含量降幅更大,MDA、 O_(2)^(-·)和H_(2)O_(2)含量增幅更大,二者间SOD、POD、APX和CAT活性差异不显著。干旱胁迫下,抗旱品种ZDM5430的抗氧化基因GPX4、CAT、Cu/Zn-SOD、2-Cys POD、Cyt-APX和DHAR表达水平高于干旱敏感品种IL-12;复水后,ZDM5430和IL-12各生理指标均表现出不同程度的补偿效应,RWC和Chl含量升高,MDA含量、 O_(2)^(-·)含量、H_(2)O_(2)含量、SOD活性、POD活性、APX活性和CAT活性下降,GPX4、CAT、Cu/Zn-SOD、2-Cys POD、Cyt-APX和DHAR基因表达量均显著下降。Drought is the main environmental stress factor affecting barley production.In order to understand the changes of antioxidant enzyme activity and related gene expression in barley under drought stress,a drought-resistant barley variety ZDM5430 and a drought-sensitive barley variety IL-12 were used as materials.The seedlings were treated by drought for 21 days and then rewatered for 7 days to study the differences of antioxidant enzyme activity and gene expression in leaves of two barley varieties.The results showed that compared with the control,the relative water content(RWC)and chlorophyll(Chl)content of the leaves decreased significantly under drought stress;the contents of malondialdehyde(MDA),superoxide anion radical( O_(2)^(-·))and hydrogen peroxide(H_(2)O_(2))increased significantly,and the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX)and catalase(CAT)increased significantly.Compared with the drought resistant variety ZDM5430,the sensitive variety IL-12 showed a greater decrease in RWC and Chl content under drought stress,while the MDA, O_(2)^(-·),and H_(2)O_(2) contents increase.There was no significant difference in SOD,POD,APX and CAT activities between the two varieties.Under drought stress,the expression levels of antioxidant genes GPX4,CAT,Cu/Zn SOD,2-Cys POD,Cyt-APX and DHAR in drought resistant variety ZDM5430 were higher than those in drought sensitive variety IL-12.After rewatering,ZDM5430 and IL-12 showed varying degrees of compensatory effects on various physiological indicators,with an increase in RWC and Chl content and a decrease in MDA content, O_(2)^(-·)content,H_(2)O_(2) content,SOD activity,POD activity,APX activity,and CAT activity.Meanwhile,the expression levels of GPX4,CAT,Cu/Zn SOD,2-Cys POD,Cyt-APX,and DHAR genes were significantly reduced.

关 键 词：大麦 干旱胁迫 抗氧化系统 基因表达 

分 类 号：S512.3[农业科学—作物学] S330