Effect of sodium nitroprusside on the microrheological properties of red blood cells in different media  

作　　者：Petr Ermolinskiy Matvey Maksimov Andrei Lugovtsov Alexey Muravyov Irina Tikhomirova Alexander Priezzhev 

机构地区：[1]Faculty of Physics,M.V.Lomonosov Moscow State University Moscow 119991,Russia [2]Faculty of Physical Education K.D.Ushinsky Yaroslavl State Pedagogical University Yaroslavl 150000,Russia [3]Faculty of Natural Sciences K.D.Ushinsky Yaroslavl State Pedagogical University Yaroslavl 150000,Russia

出　　处：《Journal of Innovative Optical Health Sciences》2024年第5期23-31,共9页创新光学健康科学杂志（英文）

基　　金：supported by the Russian Science Foundation grant(No.22-15-00120);supported by the grant(No.21-2-10-59-1)from the Foundation for the Development of Theoretical Physics and Mathematics BASIS.

摘　　要：Red blood cell(RBC)aggregation as well as their deformation significantly affects blood microrheology.These processes depend on various factors,one of which is concentration of the nitric oxide,one of the main signaling molecule in the bloodstream.The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells(RBCs)in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro.Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs,respectively.The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100,and 200M,while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation.With washed RBCs in dextran solution,the addition of sodium nitroprusside(in the concentrations of 100,and 200M)resulted in decreased aggregation and increased deformation.These-ndings aid in our understanding of nitric oxide's effect on RBC microrheological properties.

关 键 词：Sodium nitroprusside nitric oxide red blood cells RBC aggregation diffuse light scattering laser diffractometer 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]