Imaging of human parafoveal area with large field of view in adaptive optics line scanning ophthalmoscope  

作　　者：Wen Kong Yiwei Chen Guohua Shi Yi He 

机构地区：[1]Department of Biomedical Engineering,University of Science and Technology of China,Hefei 230041,P.R.China [2]Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,P.R.China [3]Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,P.R.China [4]Key Laboratory of Myopia of State Health Ministry,and Key Laboratory of Visual Impairment and Restoration of Shanghai,Shanghai 200003,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第6期41-51,共11页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China under Grant No.62075235,National Key R&D Program of China under Grant No.2021YFF0700700;Gusu Innovation and Entrepreneurship Leading Talents in Suzhou City under Grant No.ZXL2021425;Youth Innovation Promotion Association of the Chinese Academy of Sciences under Grant No.2019320;Innovation of Scientific Research Strategic Priority Research Program of the Chinese Academy of Sciences under Grant No.XDA15021304.

摘　　要：The parafoveal area,with its high concentration of photoreceptors andfine retinal capillaries,is crucial for central vision and often exhibits early signs of pathological changes.The current adaptive optics scanning laser ophthalmoscope(AOSLO)provides an excellent tool to acquire accurate and detailed information about the parafoveal area with cellular resolution.However,limited by the scanning speed of two-dimensional scanning,thefield of view(FOV)in the AOSLO system was usually less than or equal to 2,and the stitching for the parafoveal area required dozens of images,which was time-consuming and laborious.Unfortunately,almost half of patients are unable to obtain stitched images because of their poorfixation.To solve this problem,we integrate AO technology with the line-scan imaging method to build an adaptive optics line scanning ophthalmoscope(AOLSO)system with a larger FOV.In the AOLSO,afocal spherical mirrors in pairs are nonplanar arranged and the distance and angle between optical elements are optimized to minimize the aberrations,two cylinder lenses are orthogonally placed before the imaging sensor to stretch the point spread function(PSF)for sufficiently digitizing light energy.Captured human retinal images show the whole parafoveal area with 55FOV,60 Hz frame rate and cellular resolutions.Take advantage of the 5FOV of the AOLSO,only 9 frames of the retina are captured with several minutes to stitch a montage image with an FOV of 99,in which photoreceptor counting is performed within approximately 5eccentricity.The AOLSO system not only provides cellular resolution but also has the capability to capture the parafoveal region in a single frame,which offers great potential for noninvasive studying of the parafoveal area.

关 键 词：Adaptive optics line scanning retinal imaging system parafoveal capillary vasculature. 

分 类 号：R318[医药卫生—生物医学工程] R445[医药卫生—基础医学]