大鼠血流缓慢模型的制备  

Experimental study of slow blood flow model in rats

作　　者：张勇彬 陈伟毅 阮薇 林伟国 苏佳琪 邓方阁[1] Zhang Yongbin;Chen Weiyi;Ruan Wei;Lin Weiguo;Su Jiaqi;Deng Fangge(The First Affiliated Hospital of Guangzhou Medical University/Guangzhou Institute of Respiratory Health/State Key Laboratory of Respiratory Disease/National Clinical Research Center for Respiratory Disease infrared project team,Guangzhou 510120;Department of Ultrasound,Meizhou People's Hospital,Meizhou 514031;Medical Imaging Center,Guangzhou Cadre and Talent Health Management Center,Guangzhou 510700)

机构地区：[1]广州医科大学附属第一医院/广州呼吸健康研究院/呼吸疾病全国重点实验室/国家呼吸系统疾病临床医学研究中心红外项目组,广州510120 [2]梅州市人民医院超声科,梅州514031 [3]广州市干部和人才健康管理中心医学影像中心,广州510700

出　　处：《血栓与止血学》2024年第4期169-174,共6页Chinese Journal of Thrombosis and Hemostasis

基　　金：广东省自然科学基金项目(2020A1515011370);广州市开发区创新领军人才项目(2022-L002)。

摘　　要：目的通过皮下反复注射肾上腺素、冰水游泳后观察血液流变学指标和双下肢超声结果,以构建血栓形成前的大鼠血流缓慢模型。方法选取12只健康SD大鼠,肾上腺素组(n=6)皮下注射0.8 mL/kg肾上腺素,2 h后冰水游泳5 min,注射4 h后再次注射,并禁食16 h。生理盐水组(n=6)皮下注射0.8 mL/kg生理盐水,其制备方法与肾上腺素组一致。造模完成后,行双下肢股静脉超声和血液流变学检查。结果两组大鼠双下肢均无明显肿胀。在冰水游泳第1 min内,肾上腺素组大鼠较生理盐水组兴奋(P=0.015)。超声结果显示,两组大鼠股静脉均无明显血栓形成,但是肾上腺素组的右侧股静脉流速比生理盐水组低(P<0.001)。血液流变学结果显示,肾上腺素组大鼠的全血粘度低切(P=0.024)及血浆粘度(P=0.004)较生理盐水组高。结论通过大鼠皮下反复注射肾上腺素及冰水游泳,并利用血液流变学和超声检查,可以构建大鼠血流缓慢模型。Objective After repeated subcutaneous injection of epinephrine and swimming in ice water,the hemorheology indexes and ultrasound results of both lower limbs were observed to construct the slow blood flow model of rats before thrombosis.Methods Twelve healthy SD rats were selected,the epinephrine group(n=6)was injected subcutaneously with 0.8 mL/kg epinephrine,swam in ice water for 5 minutes 2 hours later,injected again 4 hours later,and fasted for 16 hours.The normal saline group(n=6)was injected subcutaneously with 0.8 mL/kg normal saline,and the preparation method was consistent with that of epinephrine group.After the molding was completed,the femoral veins of both lower limbs were examined by ultrasonography and hemorheology test.Result There was no significant swelling in the lower limbs of rats in both groups.After swimming in ice water for 1 min,rats in epinephrine group were more excited than those in normal saline group(P=0.015).Ultrasound results showed that there was no obvious thrombosis in the femoral vein in both groups,but the right femoral vein flow rate in the adrenalin group was lower than that in the normal saline group(P<0.001).The hemorheological results showed that the whole blood viscosity was lower(P=0.024)and the plasma viscosity was higher(P=0.004)in epinephrine group than in normal saline group.Conclusion By repeatedly injecting epinephrine subcutaneously and swimming in ice water,and by using hemorheology and ultrasound measurement,the rat model of slow blood flow can be constructed.

关 键 词：血流缓慢 肾上腺素 冰水游泳 深静脉血栓形成 

分 类 号：R331.3[医药卫生—人体生理学]