佛罗里达文昌鱼哈氏窝组织在性腺发育不同时期的形态结构变化  

Morphological and Structural Changes of Hatschek's Pit Tissue in Branchio-stoma floridae at Different Stages of Gonadal Development

作　　者：岑万 吴锐琼 杨理程 黄镇[1,2] CEN Wan;WU Rui-qiong;YANG Li-cheng;HUANG Zhen(College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China;Innovation and Transformation Center,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350108,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建中医药大学科学创新与转化中心,福建福州350108

出　　处：《福建农业科技》2024年第7期17-20,共4页Fujian Agricultural Science and Technology

基　　金：福建省教育厅中青年教师教育科研项目(JT180104);福建省自然科学基金项目项目(2019J01277)。

摘　　要：哈氏窝是文昌鱼生殖内分泌调控轴重要组成部分,为了进一步揭示文昌哈氏窝形态特征与性腺发育的关系,丰富文昌鱼的生物学基础信息,利用组织学切片及HE染色技术观察了佛罗里达文昌鱼在性腺发育不同时期哈氏窝形态变化。结果表明:佛罗里达文昌鱼在性腺刚出现及早期时,哈氏窝位于文昌鱼哈氏窝位于脊索附近,有一层薄膜相隔,呈近似对称的倒“凹”状;在性腺发育和成熟时期,哈氏窝顶部增厚,胞质清晰,两侧向窝内增大,窝内空隙变小,形状呈现矮宽;在性腺排空及退化时期,哈氏窝两侧呈细长状,窝内空间狭窄。The hatschek's pit is an important component of the reproductive endocrine regulation axis in Amphioxus.In order to further reveal the relationship between the morphological characteristics of hatschek's pit and gonadal development of Amphioxus,and enrich the biological basic information of Amphioxus,the morphological changes of hatschek's pit at different stages of gonadal development of Branchio-stoma floridae were observed by using the histological section and HE staining technique.The results showed that when the gonads of Branchio-stoma floridae appeared at the beginning and early stage of development,the hatschek's pit was located near the notochord of Amphioxus,with a thin film separating it,forming an approximately symmetrical inverted"concave"shape.During the development and mature period of gonads,the hatschek's pit had a thickened top,clear cytoplasm,enlarged on both sides towards the pit,smaller gaps within the pit,and a shorter and wider shape.During the period of gonadal emptying and degeneration,the two sides of the hatschek's pit were slender and the space inside the pit was narrow.

关 键 词：佛罗里达文昌鱼 哈氏窝 石蜡切片 组织学观察 

分 类 号：Q954.6[生物学—动物学]