通督调神针法对MCAO大鼠缺血半暗带超微结构及SNAP-25蛋白的影响  

Effects of Tongdu Tiaoshen Electroacupuncture on Ultrastructure of Ischemic Penumbra and SNAP-25 Protein in MCAO Rats

作　　者：谷诗浓 董竑麟 汤佳丽 李艺婷 黄晴 Gu Shi-nong;Dong Hong-lin;Tang Jia-li;Li Yi-ting;Huang Qing(Xiamen Huaxia University,Xiamen,Fujian,361024;Fujian Higher Institute Engineering Research Center for Biochemical Pharmaceuticals,Xiamen,Fujian,361024)

机构地区：[1]厦门华厦学院,福建厦门361024 [2]生化制药福建省高校工程研究中心,福建厦门361024

出　　处：《广西中医药大学学报》2024年第5期63-68,共6页Journal of Guangxi University Of Chinese Medicine

基　　金：福建省中青年教师教育科研项目(编号:JAT210594);厦门华厦学院科研项目(编号:HX202104、HX202103)。

摘　　要：[目的]观察通督调神针法对大脑中动脉闭塞(MCAO)模型大鼠行为学、缺血半暗带超微结构和突触体相关蛋白25(SNAP-25)的影响,探析电针疗法的神经保护机制。[方法]将40只SD大鼠随机分为空白组、假手术组、模型组和电针组,每组10只。空白组不做干预,假手术组大鼠仅接受皮肤切开及血管剥离术,模型组及电针组大鼠采用线栓法进行脑缺血模型制备。造模成功后电针组予通督调神针法干预,1次/日,每次30 min,连续干预7 d。空白组、假手术组及模型组术后在同等条件下抓取,回笼饲养。记录各组大鼠神经功能评分,采用2,3,5氯化三苯基四氮唑(TTC)染色法检测大鼠脑梗死面积,透射电镜观察大鼠脑组织缺血半暗带处超微结构,Western blot检测各组大鼠海马区SNAP-25蛋白表达水平。[结果]模型组大鼠神经功能评分明显低于假手术组和空白组(P<0.05);与模型组比较,电针组大鼠神经功能评分显著增加,差异有统计学意义(P<0.05)。模型组和电针组大鼠存在明显脑梗死区域,电针组脑梗死面积小于模型组(P<0.01)。空白组和假手术组大鼠脑组织神经元细胞结构完整,细胞核膜完整,结构清晰,整体细胞器完整;模型组大鼠脑组织神经元细胞结构受破坏,包括细胞膜不完整、细胞核肿胀、内质网减少等;与模型组比较,电针组大鼠脑组织具有较为完整的细胞膜及细胞核结构,线粒体空泡现象较少。与空白组、假手术组比较,模型组大鼠缺血半暗带脑组织的SNAP-25蛋白表达水平明显增加(P<0.01);与模型组比较,电针组大鼠缺血半暗带脑组织的SNAP-25蛋白表达水平明显减少(P<0.01)。[结论]通督调神针法可通过下调SNAP-25蛋白表达水平,抑制神经突触小体增殖及修复神经细胞结构,从而发挥神经保护作用。[Objective]To observe of the effects of Tongdu Tiaoshen Electroacupuncture(EA)on ethology,ultrastructure of ischemic semi-dark band and synaptosomal-associated protein 25(SNAP-25)in rats with middle cerebral artery occlusion(MCAO)model and explore the neuroprotective mechanism of Tongdu Tiaoshen EA.[Methods]40 SD rats were randomly divided into blank group,sham-operated group,model group and EA group,with 10 in each group.Animals in the blank group received no intervention.Animals in the sham-operated group were received skin incision and vascular stripping only,while rats in the model group and the EA group received operation for cerebral ischemia modeling with suture-occluded method.After successful modeling,the EA group was given Tongdu Tiaoshen Electroacupuncture,1 time a day,30 min each time,for 7 consecutive days.Rats in the blank,sham-operated and model groups were captured,caged and fed under equal conditions.The neurological function scores in all groups were recorded.The area of cerebral infarction was measured by 2,3,5 triphenyltetrazolium chloride(TTC)staining.The ultrastructure of the ischemic penumbra in the brain tissues was observed by transmission electron microscopy.And the level of SNAP-25 protein expression in hippocampus was measured by Western blot.[Results]The nerve function score in the model group was significantly lower than that in the sham-operated group and the blank group(P<0.05).And the score in the EA group was significantly higher than that of the model group(P<0.05).Cerebral infarction areas were found in both the model group and the EA group.But the infarction areas in the EA group were significantly smaller than that in the model group(P<0.01).The neuron cells in rat brain tissue in the blank and sham-operated groups remained integrity,with intact nuclear membrane,well-defined structure,and intact organelles.The neuron cell structure of brain tissue in the model group was damaged,including incomplete cell membranes,swollen nuclei,and reduced endoplasmic reticulum.Compared with

关 键 词：通督调神针法 大脑中动脉闭塞 脑缺血损伤 超微结构 SNAP-25 实验研究 

分 类 号：R285.5[医药卫生—中药学]