应用同步荧光光谱对比研究天麻粉与伪品  

A Comparative Study on Gastrodiae rhizoma Powder and Its Counterfeitsby Use of Synchronous Fluorescence Spectroscopy

作　　者：李雷 吴庆州 王涛 章曦 LI Lei;WU Qingzhou;WANG Tao;ZHANG Xi

机构地区：[1]南京理工大学紫金学院电子工程与光电技术学院,江苏南京210023

出　　处：《芜湖职业技术学院学报》2024年第3期27-30,59,共5页Journal of Wuhu Institute of Technology

基　　金：2021年江苏省高等学校基础科学(自然科学)面上研究项目“基于三维光谱和多阶校正的复杂多组分液体定量模型研究”(项目号:21KJD140003)。

摘　　要：精加工后的天麻粉与伪品难以肉眼区分,应用同步荧光光谱技术有助于对比研究天麻粉与伪品。使用爱丁堡公司FLS920荧光光谱仪获得天麻粉、伪品天花粉和紫茉莉粉的3维同步荧光光谱及等高线图谱,发现不同种类物质的光谱图像具有显著差异。通过进一步分析天麻粉中分别掺入不同伪品时的同步荧光光谱和等高线图谱,发现纯天麻粉溶液与掺入伪品的天麻粉溶液光谱图像有明显区别,有助于研究者轻松分辨天麻粉中是否掺入伪品。该方法操作简单,无需繁杂预处理与提纯,可实现实时在线检测。同时,3维同步荧光光谱从中药材的整体特征、全局视角研究荧光特性,降低了光谱分析难度,其等高线图谱具有指纹图谱特征,可完善我国中药材指纹图谱库信息,并为多组分的中药材鉴定提供参考依据。Against the practical problem of difficulty in distinguishing between finely processed Gastrodiae rhizoma powder and its counterfeits,a comparative study was made on Gastrodiae rhizoma powder and its counterfeits by use of synchronous fluorescence spectroscopy.The 3D synchronous fluorescence spectra and contour spectra of Gastrodiae rhizoma powder,Trichosanthis radix powder,and Mirabilis jalapa L.powder were obtained by use of FLS920 fluorescence spectrometer made by Edinburgh Opto-electronic Equipment.It is found that the spectral images of the above three materials differ significantly.Through subsequent analysis on synchronous fluorescence spectra and contour maps when different counterfeits are mixed into the Gastrodiae rhizoma powder,the results show that there are obvious differences between the spectral images of the pure Gastrodiae rhizoma powder solution and the Gastrodiae rhizoma powder solution with counterfeits added.This experimental method is easy to conduct real-time online detection with no need in complex preprocessing and purification.Meanwhile,the 3D synchronous fluorescence spectroscopy studies the fluorescence characteristics of traditional Chinese medicine in view of their overall characteristics from comprehensive perspective,and reduces the difficulty in spectral analysis.Its contour map has the characteristics of fingerprint,which can enrich the information of fingerprint database of traditional Chinese medicinal materials,and provide reference for the identification of multi-component traditional Chinese medicinal materials.

关 键 词：光谱学 同步荧光 天麻粉 3维同步荧光光谱 光谱分析 中药材 

分 类 号：O433.4[机械工程—光学工程]