基于HPLC-UV-QDa检测不同剂型中噻唑膦和吡虫啉  

Determination of fosthiazate and imidacloprid in variety formulations based on HPLC-UV-QDa

作　　者：顾爱国[1] 陈昌旸 刘凤琪 李雪 冯晨 王胜录 肖苏煜 杨松 GU Aiguo;CHEN Changyang;LIU Fengqi;LI Xue;FENG Chen;WANG Shenglu;XIAO Suyu;YANG Song(Jiangsu Institute of Product Quality Supervision and Inspection,Nanjing221111,China;College of Plant Protection,Shandong Agricultural University,Shandong Tai'an271018,China;College of Agriculture,Yangtze University,Hubei Jingzhou434000,China;College of Plant Protection,Nanjing Agricultural University,Nanjing221111,China)

机构地区：[1]江苏省产品质量监督检验研究院,南京221111 [2]山东农业大学植物保护学院,山东泰安271018 [3]长江大学农学院,湖北荆州434000 [4]南京农业大学植物保护学院,南京221111

出　　处：《现代农药》2024年第5期64-69,共6页MODERN AGROCHEMICALS

基　　金：江苏省农业科技自主创新项目[CX(22)3017]:江苏省市场监督管理局科技计划项目(KJ2023011):江苏省市场监督管理局科技计划重点研发项目(KJ2024003)。

摘　　要：建立了高效液相色谱-紫外检测器-质谱(HPLC-UV-QDa)测定5种不同剂型产品中噻唑膦和吡虫啉含量的分析方法。样品以乙腈+水(0.05%甲酸)为流动相,采用C18色谱柱进行梯度洗脱,结合紫外和质谱串联检测器进行定性和定量分析。研究结果显示,在质量浓度0.05~50 mg/L,噻唑膦、吡虫啉的线性关系良好,R2分别为0.9992~0.9999和0.9991~0.9996。在0.5、50、500 mg/kg等3个添加水平下,噻唑膦、吡虫啉的平均回收率分别为90.2%~103.9%和94.7%~104.1%,相对标准偏差(RSD)分别为1.04%~6.69%和1.27%~5.88%。噻唑膦和吡虫啉在5种基质中无明显基质效应,检出限分别为0.005和0.01 mg/L,定量限分别为0.02和0.04 mg/kg。方法灵敏度高、稳定性好、准确度高,适用于不同剂型产品中噻唑膦和吡虫啉的分析。A high performance liquid chromatography-ultraviolet detector-mass spectrometry(HPLC-UV-QDa)method was developed for the determination of fosthiazate and imidacloprid in 5 different formulations.The samples were analyzed by gradient elution separation,using acetonitrile-water(0.05%formic acid)as the mobile phase on C18 column.The results showed that the linear relations were good at the concentration of 0.05-50 mg/L,R^(2) of fosthiazate and imidacloprid were 0.9992-0.9999 and 0.9991-0.9996,respectively.Under the level of 0.5,50,500 mg/kg,the recoveries of fosthiazate and imidacloprid were 90.2%-103.9%and 94.7%-104.1%,and the relative standard deviations were 1.04%-6.69%and 1.27%-5.88%,respectively.The matrix effects of fosthiazate and imidacloprid were non-obvious in 5 typical substrates,and LOD were 0.005 and 0.01 mg/L,LOQ were 0.02 and 0.04 mg/kg.The method was suitable for the analysis of fosthiazate and imidacloprid in different formulations.

关 键 词：高效液相色谱-紫外检测器-质谱 噻唑膦 吡虫啉 基质效应 分析 

分 类 号：TQ450.7[化学工程—农药化工]