饲料中添加丁酸梭菌或丁酸钠对大口黑鲈幼鱼肠道短链脂肪酸含量、消化酶活性与抗氧化指标以及肠道结构的影响  

Effects of Dietary Supplementation with Clostridium butyrate or Sodium Butyrate on Intestinal Short-Chain Fatty Acids Contents,Digestive Enzymes Activities,Antioxidant Indexes and Intestinal Structure of Juvenile Largemouth Bass

作　　者：侯冬强 胡俊茹 陈冰 彭凯 李培佳 黄文 曹俊明[3] 赵红霞 HOU Dongqiang;HU Junru;CHEN Bing;PENG Kai;LI Peijia;HUANG Wen;CAO Junming;ZHAO Hongxia(Collaborative Innovation Center of Aquatic Sciences,Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Guangzhou Fishtech Fisheries Science and Technology Co.,Ltd.,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院动物科学研究所,广东省畜禽育种与营养研究重点实验室,农业农村部华南动物营养与饲料重点实验室,广东省农业科学院水产协同创新中心,广州510640 [2]广东海洋大学水产学院,湛江524088 [3]广东省农业科学院,广州510640 [4]广州飞禧特生物科技有限公司,广州510640

出　　处：《动物营养学报》2024年第9期5883-5894,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31402307,31902388);广东省科技计划项目(KTP20210322);广州市科技计划项目(2023E04J0407)。

摘　　要：本试验旨在研究饲料中添加丁酸梭菌(CB)或丁酸钠(SB)对大口黑鲈(Micropterus salmoides)幼鱼肠道短链脂肪酸含量、消化酶活性与抗氧化指标以及肠道结构的影响。试验配制3种等氮(50%)和等脂(9%)的饲料,分别为基础饲料(对照组)、基础饲料+10 mL/kg CB(CB组)、基础饲料+1.0 g/kg SB(SB组)。试验挑选360尾大口黑鲈[(5.02±0.01)g],随机分为3组,分别饲喂3种饲料,每组3个重复,每个重复40尾鱼。试验期为56 d。结果显示:1)与对照组相比,CB组终末体重和特定生长率显著提高(P<0.05),SB组无显著差异(P>0.05)。2)与对照组相比,CB组肠道中乙酸含量显著升高(P<0.05),CB组和SB组肠道丙酸、异丁酸和异戊酸含量显著提高(P<0.05)。3)与对照组相比,饲料中添加CB或SB可显著提高肠道中淀粉酶、胰蛋白酶和脂肪酶活性(P<0.05),CB组肠道中碱性磷酸酶活性显著提高(P<0.05),SB组肠道中钠钾ATP酶活性显著提高(P<0.05)。4)CB组和SB组肠道中过氧化氢酶活性显著高于对照组(P<0.05)。与对照组相比,SB组肠道中丙二醛含量显著降低(P<0.05),CB组肠道中总抗氧化能力显著提高(P<0.05)。5)与对照组相比,CB组中肠绒毛宽度显著增加(P<0.05),减轻了肠道炎症反应。综上所述,饲料中添加CB显著提高了大口黑鲈幼鱼的生长性能,添加CB或SB增加了大口黑鲈肠道短链脂肪酸含量,显著提高了肠道消化酶活性与抗氧化指标以及改善肠道结构,与SB组相比,添加10 mL/kg的CB对提高大口黑鲈生长性能、肠道抗氧化指标以及增加肠道绒毛宽度效果更佳。The purpose of this experiment is to study the effects of dietary supplementation with Clostridium butyrate(CB)or sodium butyrate(SB)on intestinal short-chain fatty acids contents,digestive enzymes activities,antioxidant indexes and intestinal structure of juvenile largemouth bass.Three iso-nitrogen(50%)and iso-lipid(9%)diets were prepared,which were the base diet(CON group),the base diet+10 mL/kg CB(CB group)and the base diet+1.0 g/kg SB(SB group),respectively.A total of 360 fish[(5.02±0.01)g]were randomly divided into 3 groups with 3 replicates per group and 40 fish per replicate.The experiment period was 56 days.The results showed as follows:1)compared with the control group,the final weight and specific growth rate in CB group were significantly increased(P<0.05),but there was no significant difference in SB group(P>0.05).2)Compared with the control group,the intestinal acetic acid content in CB group was significantly increased(P<0.05),and the contents of propionic acid,isobutyric acid and isovaleric acid in CB and SB groups were significantly increased(P<0.05).3)Compared with the control group,dietary CB and SB supplementation significantly increased the activities of amylase,trypsin and lipase in the intestine(P<0.05),CB group significantly increased the activity of alkaline phosphatase in the intestine(P<0.05),and SB group significantly increased the activity of sodium-potassium-ATPase(P<0.05).4)The intestinal catalase activity in CB and SB groups was significantly higher than that in control group(P<0.05).Compared with the control group,the malondialdehyde content in SB group was significantly decreased(P<0.05),and the total antioxidant capacity in CB group was significantly increased(P<0.05).5)Compared with the control group,the villi width in the middle intestine in CB group was significantly increased(P<0.05),which alleviated intestinal inflammation.In conclusion,dietary CB significantly improve the growth performance of largemouth bass juvenile fish,and the addition of CB or SB significantly increas

关 键 词：大口黑鲈 丁酸梭菌 丁酸钠 消化酶 抗氧化酶 肠道结构 

分 类 号：S963[农业科学—水产养殖]