欧前胡素对高脂型蛋氨酸-胆碱缺乏饲粮诱导的非酒精性脂肪性肝病小鼠血清生化指标和肝脏的影响  

Effects of Imperatorin on Serum Biochemical Parameters and Liver of Mice with Non-Alcoholic Fatty Liver Disease Induced by Methionine and Choline Deficient High-Fat Diet

作　　者：李仕韦 邱鹏 孟凡舜 夏榕鸽 李士泽[1] LI Shiwei;QIU Peng;MENG Fanshun;XIA Rongge;LI Shize(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Key Laboratory of Conservation and Utilization of Subtropical Agricultural Biological Resources,College of Animal Science and Technology,Guangxi University,Nanning 530005,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]广西大学动物科学技术学院,亚热带农业生物资源保护与利用国家重点实验室,南宁530005

出　　处：《动物营养学报》2024年第9期6027-6037,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：科技创新2030-重大项目“优质猪新品种设计与培育”(2023ZD0404606);国家自然科学基金面上项目(31972637)。

摘　　要：本试验旨在研究欧前胡素(IMP)对高脂型蛋氨酸-胆碱缺乏饲粮(MCD-HFD)引起的小鼠非酒精性脂肪性肝病(NAFLD)的影响。将50只9周龄雄性C57BL/6小鼠随机分为5组,每组10只。MCS-LFD组(对照组)饲喂蛋氨酸-胆碱充分低脂饲粮(MCS-LFD),MCD-HFD组(模型组)、MCD-HFD+IMP25组、MCD-HFD+IMP50组和MCD-HFD+IMP100组小鼠饲喂MCD-HFD,连续喂养8周。各组均在喂养4周后对小鼠进行灌胃处理,连续4周。MCD-HFD+IMP25组、MCD-HFD+IMP50组和MCD-HFD+IMP100组小鼠分别灌胃25、50和100 mg/(kg·d)IMP,MCS-LFD组和MCD-HFD组小鼠灌胃等量生理盐水。饲喂8周后处死,取血清检测天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性和甘油三酯(TG)、总胆固醇(TC)含量;通过苏木精-伊红(HE)染色以及Masson染色检测肝脏组织脂肪变性和纤维化;称量肝脏湿重,计算肝脏指数;通过Western blot法检测肝脏中肉碱棕榈酰基转移酶1A(CPT1A)的蛋白表达量。结果显示:1)与MCS-LFD组相比,MCD-HFD组小鼠体重和增重显著降低(P<0.05),肝脏湿重显著增加(P<0.05),肝脏指数极显著升高(P<0.01),血清中AST、ALT活性和TG、TC含量均极显著升高(P<0.01),肝脏中CPT1A的蛋白表达量极显著降低(P<0.01)。2)切片结果显示,与MCS-LFD组相比,MCD-HFD组小鼠肝细胞排列紊乱,发生气球样变性,出现大量脂肪性空泡,且肝细胞周围纤维化。3)与MCD-HFD组相比,MCD-HFD+IMP50组小鼠的体重和增重显著升高(P<0.05),肝脏湿重显著降低(P<0.05),肝脏指数极显著降低(P<0.01),血清AST和ALT活性极显著下降(P<0.01),TG和TC含量显著下降(P<0.05),肝脏中CPT1A的蛋白表达量显著升高(P<0.05)。4)与MCD-HFD组相比,MCD-HFD+IMP100组小鼠体重无显著变化(P>0.05),肝脏湿重和肝脏指数均极显著降低(P<0.01),血清AST、ALT活性和TG、TC含量均极显著下降(P<0.01)。5)与MCD-HFD组相比,IMP处理能够减少肝脏中空泡数量,改善肝细胞气球样变性和纤维化程度,且呈剂�The aim of this experiment was to investigate the effects of imperatorin(IMP)on nonalcoholic fatty liver disease(NAFLD)induced by methionine and choline deficient high-fat diet(MCD-HFD)in mice.Fifty 9-week-old male C57BL/6 mice were randomly divided into 5 groups with 10 mice in each group.Mice in MCS-LFD group(control group)fed with methionine and choline sufficient low-fat diet(MCS-LFD),and those in MCD-HFD group(model group),MCD-HFD+IMP25 group,MCD-HFD+IMP50 group and MCD-HFD+IMP100 group fed with MCD-HFD for 8 weeks.Mice in all groups were treated by gavage after 4 weeks of feeding for 4 consecutive weeks.Mice in MCD-HFD+IMP25 group,MCD-HFD+IMP50 group and MCD-HFD+IMP100 group were treated by gavage with 25,50 and 100 mg/(kg·d)IMP,respectively,and mice in MCS-LFD group and MCD-HFD group were treated by gavage with equal amount of saline.After 8 weeks,the mice were sacrificed,and the serum aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activities,triglyceride(TG)and total cholesterol(TC)contents were detected;hepatic steatosis and fibrosis were detected by hematoxylin-eosin(HE)staining and Masson staining;liver wet weight was measured and liver index was calculated;the protein expression level of carnitine palmitoyl-transferase 1A(CPT1A)in the liver was detected by Western blot method.The results showed as follows:1)compared with the MCS-LFD group,the mice in the MCD-HFD group showed a significant reduction in body weight and weight gain(P<0.05);the liver wet weight(P<0.05),liver index(P<0.01),serum AST and ALT activities(P<0.01),serum TG and TC content(P<0.01)of the MCD-HFD group were significantly or extremely significantly increased;the protein expression level of CPT1A in the liver of the MCD-HFD group was extremely significantly decreased(P<0.01).2)The results of sections showed that compared with the MCS-LFD group,the MCD-HFD group had disordered arrangement of hepatocytes,ballooning degeneration,many fatty vacuoles,and fibrosis around hepatocytes.3)Compared with the MCD-HFD group,the b

关 键 词：非酒精性脂肪性肝病 欧前胡素 脂肪变性 肉碱棕榈酰基转移酶1A 

分 类 号：R93[医药卫生—生药学]