车前草提取物对小鼠急性酒精性肝损伤的保护作用  

Protective Effect of Plantain Extract against Acute Alcohol-induced Liver Injury in Mice

作　　者：李东海 杨娟 王瑜 张朝举 杨小生 李祖辉 李立郎 LI Donghai;YANG Juan;WANG Yu;ZHANG Chaoju;YANG Xiaosheng;LI Zuhui;LI Lilang(College of Pharmacy,Guizhou Medical University,Guiyang 550025,China;Guizhou Natural Products Research Center,Guiyang 550014,China;Guizhou Zhongyi Food Limited Company,Guiyang 550025,China)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州省天然产物研究中心,贵州贵阳550014 [3]贵州中意食品有限责任公司,贵州贵阳550025

出　　处：《现代食品科技》2024年第9期20-26,共7页Modern Food Science and Technology

基　　金：贵州省科技计划项目(黔科合成果[2022]一般030);贵州省科技创新能力建设专项(黔科合服企[2020]4013号);贵州省教育厅高等学校特色重点实验室建设项目(黔教合KY字[2020]018号)

摘　　要：该实验研究了车前草提取物(Plantain Extract,PE)对急性酒精性肝损伤小鼠的保护作用。通过灌胃体积分数52%的食用酒精建立小鼠急性酒精性肝损伤模型,测定小鼠肝脏指数、血清谷丙转氨酶(Alanine Aminotransferase,ALT)、谷草转氨酶(Aspartate Aminotransferase,AST)活性和甘油三酯(Triglycerides,TG)的含量,以及肝组织中超氧化物歧化酶(Superoxide Dismutase,SOD)、过氧化氢酶(Catalase,CAT)活性和总谷胱甘肽(Total Glutathione,T-GSH)、丙二醛(Malondialdehyde,MDA)的含量,并对小鼠肝组织进行病理学观察。结果显示,与模型组相比,阳性对照组和给药组小鼠肝脏指数显著下降(P<0.01),给药组最大降幅为20.18%,高剂量组血清中ALT、AST和TG水平显著降低27.29%、36.03%、45.69%,肝组织中CAT和SOD活性、T-GSH含量均显著增加16.36%、18.42%、28.66%及MDA含量显著降低36.89%,且肝组织病理切片显示给药组小鼠肝损伤程度均有显著改善。由此可见,PE对急性酒精诱导的肝损伤具有明显的保护作用。In this study,the protective effect of plantain Extract(PE)against acute alcohol-induced liver injury was studied in mice.A mouse model of acute alcoholic liver injury was established by intragastric administration of 52%(by volume)edible alcohol.The liver index,serum alanine aminotransferase(ALT)activity,aspartate aminotransferase(AST)activity and triglyceride(TG)content,as well as superoxide dismutase(SOD)activity,catalase(CAT)activity,total glutathione(T-GSH)content,malondialdehyde(MDA)content of mouse liver tissues were determined,and pathologic observation of mouse liver tissues was performed.The results showed that compared with the model group,the liver index of the mice in the positive control group and the drug administration group decreased significantly(P<0.01),with the maximum decrease of 20.18%in the administered group,and the levels of serum ALT,AST and TG of the high-dose group were significantly reduced by 27.29%,36.03%and 45.69%,respectively,and in the liver tissues,the CAT activity,SOD activity and T-GSH content increased significantly(by 16.36%,18.42%and 28.66%,respectively),and the MDA content decreased significantly(by 36.89%).The histopathological analysis of the liver sections showed significant improvement in the degree of hepatic injury for the administered mice.Accordingly,PE has a significant protective effect against acute alcohol-induced liver injury.

关 键 词：车前草提取物 急性酒精肝损伤 保护作用 抗氧化 病理切片 

分 类 号：R285.5[医药卫生—中药学]