电针治疗调节miR-183对噪声性耳聋模型耳蜗毛细胞VEGF表达的影响  

Electroacupuncture treatment regulates the effect of miR-183 on VEGF expression in cochlear hair cells of noise-induced hearing loss model

作　　者：王荣国[1] 高洁[1] 张慧平[1] 屈永涛[1] WANG Rongguo;GAO Jie;ZHANG Huiping;QU Yongtao(Department of Otolaryngology,Hebei Provincial People′s Hospital,Shijiazhuang 050051,Hebei,China)

机构地区：[1]河北省人民医院耳鼻喉科,河北石家庄050051

出　　处：《皖南医学院学报》2024年第5期409-413,共5页Journal of Wannan Medical College

基　　金：河北省医学科学研究课题计划项目(20210800)。

摘　　要：目的:探讨电针治疗调节miR-183对噪声性耳聋(NIHL)模型耳蜗毛细胞血管内皮生长因子(VEGF)表达的影响与作用机制。方法:将雄性SD大鼠随机均分为空白对照组、NIHL组、NIHL+针刺组、NIHL+针刺+腺病毒(AAV)-NC组和NIHL+针刺+AAV-sp-miR-183组。针刺组取中渚穴进行刺激。利用AAV载体在NIHL大鼠中敲低miR-183。各组大鼠进行听觉脑干反应(ABR)评估与毛细胞计数。qRT-PCR分析大鼠耳蜗组织miR-183水平。蛋白质免疫印迹测定VEGF水平。结果:与对照组相比,NIHL组耳蜗组织中miR-183的水平降低,ABR阈值差与毛细胞的缺失率升高,耳蜗毛细胞VEGF表达的水平升高(P<0.05)。与NIHL组相比,NIHL+针刺组耳蜗组织中miR-183的水平上升,ABR阈值差与毛细胞的缺失率降低,耳蜗毛细胞VEGF表达的水平升高(P<0.05)。NIHL+针刺+AAV-sp-miR-183组与NIHL+针刺+AAV-NC组相比,miR-183的水平降低,ABR阈值差与毛细胞的缺失率升高,耳蜗毛细胞VEGF表达的水平降低(P<0.05)。结论:电针治疗可以通过上调miR-183进而促进NIHL大鼠耳蜗毛细胞VEGF的表达,降低噪音诱导的NIHL大鼠ABR阈值差增高与毛细胞损伤。Objective:To investigate the effect and mechanism of electroacupuncture treatment in regulation of miR-183 on VEGF expression in cochlear hair cells in animal models of noise-induced hearing loss(NIHL).Methods:Male SD rats were randomly divided into control group,NIHL group,NIHL+acupuncture group,NIHL+acupuncture+AAV-NC group and NIHL+acupuncture+AAV-sp-miR-183 group.Rats in the acupuncture were stimulated via Zhongzhu point.MiR-183 was knocked down in NIHL rats using adeno-associated virus(AAV)vectors.The auditory brainstem response(ABR)was evaluated and the hair cells were counted.qRT-PCR was used to analyze the level of miR-183 in rat cochlear tissue.VEGF levels were determined by Western blot.Results:Compared with the control group,the level of miR-183 in cochlear tissue of NIHL group was significantly decreased,whereas the shift of ABR threshold and the loss rate of hair cells as well as the expression level of VEGF in cochlear hair cells were notably increased(all P<0.05).Markedly increased level of miR-183,yet decreased shift of ABR threshold and the loss rate of hair cells and escalated VEGF expression level were seen in cochlear tissue in NIHL+acupuncture group compared to NIHL group(all P<0.05).Comparison of NIHL+acupuncture+AAV-NC group with NIHL+acupuncture+AAV-sp-miR-183 group indicated that miR-183 level was significantly down-regulated,the shift of ABR threshold and the loss rate of hair cells were notably increased,yet VEGF expression level in cochlear hair cells was markedly attenuated(all<0.05).Conclusion:Electroacupuncture could promote VEGF expression in cochlear hair cells of NIHL rats by up-regulating miR-183,and reduce the increase of ABR threshold shift and hair cell damage in the NIHL rat models.

关 键 词：电针治疗 噪声性耳聋 miR-183 耳蜗毛细胞 血管内皮生长因子 

分 类 号：R764.433[医药卫生—耳鼻咽喉科] R.332[医药卫生—临床医学] R245