微柱-酶法在不规则抗体筛查中的应用研究  

Application of Microcolumn Enzyme Method in Irregular Antibody Screening

作　　者：庞德[1] 陈寅凤 向娟 梁俊瑶 PANG De;CHEN Yinfeng;XIANG Juan;LIANG Junyao(Department of Blood Transfusion,The First People's Hospital of Yulin,Yulin 537000,China)

机构地区：[1]广西玉林市第一人民医院输血科,玉林537000

出　　处：《中国医药指南》2024年第28期119-122,共4页Guide of China Medicine

摘　　要：目的研究传统微柱凝胶法与微柱-酶法在不规则抗体筛查中的敏感度及特异度,为提高不规则抗体筛查检出率提供循证依据。方法选择2022年11月至2023年12月在玉林市第一人民医院就诊的输血不良反应患者60例为研究对象,分别用传统微柱凝胶法及微柱-酶法进行不规则抗体筛查的对比研究,比较两种筛查方法的不规则抗体检出率、不规则抗体效价;同时采用倍比稀释法检查两种方法测试不规则抗体的最高滴度,采用excel表格AVERAGE函数公式计算平均滴度;另外选取同时期在该院体检中健康人群60名作为不规则抗体筛查阴性对照组,分别评价两种方法对不规则抗体检查的敏感度及特异度。结果微柱-酶法对不规则抗体检出率高于传统微柱凝胶法[8(13.3%)vs.18(30.0%),P<0.05],微柱-酶法平均滴度高于传统微柱凝胶法[(1∶18)vs.(1∶42),P<0.05]。传统微柱凝胶法及微柱-酶法敏感度差异无统计学意义(89.5%vs.90.8%,P>0.05);传统微柱凝胶法及微柱-酶法特异度(78.9%vs.80.2%,P>0.05)。结论微柱-酶法对不规则抗体有更高的检出滴度,传统酶法及微柱-酶法对不规则抗体检测敏感度及特异度无统计学差异,均能满足临床输血对于不规则抗体的检测需求。Objective To study the sensitivity and specificity of traditional microcolumn gel method and microcolumn enzyme method in irregular antibody screening,so as to provide an evidence-based basis for improving the detection rate of irregular antibody screening.Methods A total of 60 patients with adverse blood transfusion reactions attending the First People's Hospital of Yulin from November 2022 to December 2023 were selected for the study,and a comparative study of irregular antibody screening was carried out by the traditional microcolumn gel method and microcolumn-enzyme method respectively,comparing the detection rate of irregular antibody and the potency of irregular antibody of the two screening methods;at the same time,the doubled dilution method was used to check the highest titer of the irregular antibody of the two methods.At the same time,the highest titer of irregular antibody was examined by the doubling dilution method,and the average titer was calculated by using the formula of AVERAGE function in excel sheet;in addition,60 healthy people were selected as the negative control group of irregular antibody screening during the same period of medical checkups in the hospital,and the sensitivities and specificities of the two methods of irregular antibody screening were evaluated respectively.Results The detection rate of irregular antibodies was higher in the microcolumn-enzyme method than in the traditional microcolumn gel method[8(13.3%)vs.18(30.0%),P<0.05],and the average titer of the microcolumn-enzyme method was higher than that of the traditional microcolumn gel method[(1:18)vs.(1:42),P<0.05].There was no statistically significant difference between the sensitivity of the traditional microcolumn gel method and the microcolumn-enzyme method(89.5%vs.90.8%,P>0.05);the specificity of the traditional microcolumn gel method and the microcolumn-enzyme method(78.9%vs.80.2%,P>0.05).Conclusions The micro column enzyme method has a higher detection titer for irregular antibodies,and there is no statistically si

关 键 词：微柱法 微柱-酶法 不规则抗体 敏感度 特异度 

分 类 号：R446.11[医药卫生—诊断学] R457.1[医药卫生—临床医学]