甘蓝型油菜NHX基因家族鉴定及其在盐胁迫下的表达分析  

Identification and Expression under Salt Stress of NHXs in Brassica napus L.

作　　者：安蓉 李永红[1] 张振兰[1] 李建厂[1] 李阿立 唐改娟 穆建新[1] AN Rong;LI Yonghong;ZHANG Zhenlan;LI Jianchang;LI Ali;TANG Gaijuan;MU Jianxin(Hybrid Rapeseed Research Center of Shaanxi Province,Yangling,Shaanxi 712100,China)

机构地区：[1]陕西省杂交油菜研究中心,陕西杨凌712100

出　　处：《福建农业学报》2024年第6期643-651,共9页Fujian Journal of Agricultural Sciences

基　　金：陕西省自然科学基础研究计划项目(2024JC-YBQN-0151);中央引导地方科技发展专项资金项目(2024ZY-CGZY-21);陕西省农业科技创新驱动项目(NYKJ-2022-5)。

摘　　要：【目的】系统鉴定甘蓝型油菜(Brassica napus L.)Na+/H+逆向转运蛋白(Na+/H+antiporter, NHX)家族成员,筛选盐胁迫相关候选基因。【方法】利用公开的甘蓝型油菜品种中双11的基因组序列,通过同源比对的方法,在全基因组范围内获得NHX候选基因家族基因及蛋白序列,分析其理化性质、进化关系及盐胁迫下基因表达模式。【结果】共鉴定出21个甘蓝型油菜NHX基因家族成员,其氨基酸数量为71~1 265 aa,等电点pI为5.54~7.68,内含子为0~24个;其中BnNHX1基因定位于细胞质膜,BnNHX5、BnNHX12定位于细胞膜及液泡膜,其余基因均只定位在液泡。NHX基因家族可分为3个亚家族,分布在11条染色体上,顺式作用元件分析表明NHX基因家族含有多种非生物胁迫响应元件。qRT-PCR结果发现BnNHXs基因受盐胁迫后,多数基因表达量上调,且叶片上的表达量整体高于根部,其中以BnNHX2、BnNHX6、BnNHX8、BnNHX11和BnNHX19等5个基因表达量变化相对较大。【结论】BnNHX2、BnNHX6、BnNHX8、BnNHX11和BnNHX19等5个基因可作为油菜的耐盐候选功能基因,为下一步利用NHX基因开展油菜耐盐育种及耐盐分子机制研究奠定了基础。Objective Members of the Na+/H+antiporter(NHX)family in Brassica napus L.were identified,and genes related to salt stress screened.Methods The genome of B.napus cv.Zhongshuang 11 was used to obtain the candidate genes,and NHXs sequences by homologous alignment.Physicochemical properties,evolutionary relationship,and expressions under salt stress of the genes were analyzed.Results Twenty-one BnNHXs were identified with 71 to 1265 amino acids,an isoelectric(pI)ranging from 5.54 to 7.68,and 0 to 24 introns.Among the BnNHXs,BnNHX1 was in the plasma membrane,BnNHX5 and BnNHX12 in the cell and vacuole membranes,and the remainders in the vacuole only.Phylogenetically,NHXs could be divided into 3 subfamilies scattered on 11 chromosomes containing a variety of abiotic stress response elements.The qRT-PCR expressions on most BnNHXs were upregulated under salt stress,with overall higher expression in the leaves than in the roots.Notably,BnNHX2,BnNHX6,BnNHX8,BnNHX11,and BnNHX19 had relatively more pronounced changes in the expression.Conclusion Five NHXs,namely,BnNHX2,BnNHX6,BnNHX8,BnNHX11,and BnNHX19,could be the candidate genes for breeding salt-tolerant new varieties as well as for studying molecular mechanisms of rapeseed.

关 键 词：甘蓝型油菜 NHX基因家族 基因表达 盐胁迫 

分 类 号：Q78[生物学—分子生物学] S565.4[农业科学—作物学]