多花黄精种子生理后熟过程的生理变化及基因表达模式分析  

Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment

作　　者：苏海兰[1] 牛雨晴 成建华 朱雁鸣 陈宏[1] 郑梅霞[1] 黄贤贵[3] 朱育菁[1] SU Hailan;NIU Yuqing;CHENG Jianhua;ZHU Yanming;CHEN Hong;ZHENG Meixia;HUANG Xiangui;ZHU Yujing(Institute of Crop Sciences,Fujian Academy of Agricultural Sciences/Fujian Germplasm Resources Center,Fuzhou,Fujian 350003,China;Shaowu Forest Bureau,Nanping,Fujian 354000,China;Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350012,China)

机构地区：[1]福建省农业科学院作物研究所/福建省种质资源中心,福建福州350003 [2]福建省南平市邵武市林业局,福建南平354000 [3]福建省农业科学院植物保护研究所,福建福州350012

出　　处：《福建农业学报》2024年第6期662-670,共9页Fujian Journal of Agricultural Sciences

基　　金：福建省科技重大专项(2022NZ029017);福建省科技计划公益类专项(2023R1032003);福建省农业科学院英才项目(YC2021002);福建省农业科学院科技创新团队建设项目(2022KF01-1);南平市科技计划项目(N2022T021)。

摘　　要：【目的】探明多花黄精种子生理后熟过程中生理生化变化规律及差异基因表达情况。【方法】以多花黄精种子生理后熟过程6个阶段的种子为材料,采用化学测定方法,分析每个阶段的超氧化物歧化酶、α淀粉酶、β淀粉酶、可溶性蛋白、粗脂肪、可溶性糖含量。采用高通量转录组测序技术,对6个阶段的差异表达基因进行分析,并用qRT-PCR法对关键差异基因的表达量进行验证。【结果】(1)多花黄精种子生理后熟过程中,可溶性糖含量在前4个时期逐渐增高后,在后两个时期稍有下降;粗脂肪、蛋白质整体呈下降趋势;α-淀粉酶活性及β-淀粉酶活性呈现增强趋势。(2)多花黄精种子生理后熟过程中,蔗糖和淀粉代谢途径、激素信号转导通路等显著富集差异表达基因。【结论】多花黄精种子生理后熟过程中,淀粉等营养物质的代谢、转化与内源激素的协调作用,促进多花黄精种子的生理后熟。Objective Physiobiochemical changes and differentially expressed genes in Polygonatumcyrtonema Hua seeds after a post-ripening treatment were studied.Method Contents of superoxide dismutase,α-amylase,β-amylase,soluble protein,crude fat,and soluble sugar at 6 seed development stages were monitored.Differentially expressed genes were analyzed with high-throughput sequencing followed by a qRT-PCR verification.Result(1)The post-ripening treatment raised the soluble sugar content in the seeds gradually in the initial 4 stages and slightly declined in the last two stages,while induced a declining trend on the crude fat and protein.Meanwhile,the activities ofα-amylase andβ-amylase increased.(2)Significantly enriched differentially expressed genes were observed in the sucrose and starch metabolic pathways as well as the hormone signal transduction pathway during the process.Conclusion The post-ripening treatment on the P.cyrtonema seeds brought about the conversion and metabolism of starch and other nutrients as well as endogenous hormones interactions promoting the physiological changes and dormancy release.

关 键 词：多花黄精 生理后熟 转录组测序分析 

分 类 号：R28[医药卫生—中药学]