不同光照强度对油樟1,8-桉叶素含量的影响  

Influence of different light intensities on 1,8-cineole content in Camphora longepaniculata leaves

作　　者：陈楠 柳欣 魏琴[1,2] 赵鑫 CHEN Nan;LIU Xin;WEI Qin;ZHAO Xin(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin 644000,China;Sichuan Oil Cinnamon Engineering Technology Research Center,Yibin 644000,China)

机构地区：[1]宜宾学院农林与食品工程学部,四川宜宾644000 [2]四川省油樟工程技术研究中心,四川宜宾644000

出　　处：《四川林业科技》2024年第5期49-57,共9页Journal of Sichuan Forestry Science and Technology

基　　金：基于多组学的油樟产油性能分析及其调控路径研究(412-0219019402)。

摘　　要：油樟为樟科樟属植物,其次生代谢产物油樟精油富含1,8-桉叶素,在食品、化工、医疗等领域具有广泛经济价值。植物生长过程及次生代谢受到外界环境变化的影响,而光照强度如何影响油樟精油含量及其成分变化,如何调控精油合成相关基因的表达目前尚无报道。以不同光照油樟叶片为材料,研究不同光照强度下油樟叶片精油及1,8-桉叶素含量的差异,分析1,8-桉叶素合成路径中8个关键基因的表达变化。结果表明:(1)不同光照强度影响油樟叶片形态发育及内源激素含量;(2)不同光照下油樟叶片精油含量显著差异,树阳面叶片精油含量比树阴面高2.3%。光照强度引起油樟精油1,8-桉叶素含量显著差异;(3)树阳面油樟叶片中DXR、CMK、HGMR、GPPS、NPPS、CINS、LIS基因上调表达,而HDS基因下调表达,不同光照影响萜类合成途径关键基因的表达。旨在深入分析光照对油樟精油及1,8-桉叶素含量的影响,为进一步优化油樟栽培条件,提高1,8-桉叶素含量及生物技术调控产油提供理论基础。Camphora longepaniculata is a plant belonging to the Lauraceae family,and it is famous for its essential oil which is rich in 1,8-cineole.The 1,8-cineole has extensive economic value in the fields of food,chemical industry,and medical treatment.Plant growth process and secondary metabolism are regulated by changes in external environment,but there is no report on how light intensity affects the essential oil content and its composition changes,and how to regulate the expression of essential oil synthesis-related genes.In this experiment,C.longepaniculata leaves were used as materials to study the differences in the content of essential oil and 1,8-cineole in C.longepaniculata leaves under different light intensities,and to analyze the expression changes of eight key genes in the synthesis pathway of 1,8-cineole.The results showed that:(1)Different light intensities affected the leaf morphological development and endogenous hormone content;(2)There were significant differences in the content of essential oils and 1,8-cineole in leaves of C.longepaniculata under different lighting conditions.Light intensity was beneficial to the expression of key enzyme genes in the terpenoid synthesis pathway;(3)The expression of DXR,CMK,HGMR,GPPS,NPPS,CINS,and LIS were up-regulated in sun-side leaves of C.longepaniculata,while HDS were down-regulated.The purpose of this study was to deeply analyze the effect of light on the content of essential oil and 1,8-cineole in C.longepaniculata,and to provide a theoretical basis for further optimizing the cultivation conditions of C.longepaniculata,increasing the content of 1,8-cineole and regulating oil production by biotechnology.

关 键 词：光照强度 油樟精油 1 8-桉叶素 萜类合成基因 

分 类 号：S792.23[农业科学—林木遗传育种]