抗唾液腺蛋白1抗体联合抗腮腺分泌蛋白抗体对干燥综合征的诊断价值  

Diagnostic values of anti-salivary gland protein-1 antibody combined with anti- parotid secretory protein antibody for Sj gren’ s syndrome

作　　者：杨玉淑 齐晅[1] 丁萌[1] 王炜[1] 郭惠芳[1] 高丽霞[1] YANG Yushu;QI Xuan;DING Meng;WANG Wei;GUO Huifang;GAO Lixia(Department of Rheumatology and Immunology,the Second Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第二医院风湿免疫科,石家庄050011

出　　处：《北京大学学报（医学版）》2024年第5期845-852,共8页Journal of Peking University:Health Sciences

基　　金：河北省科技厅重点研发计划民生科技专项(20377782D)。

摘　　要：目的:探讨抗唾液腺蛋白1(salivary protein 1, SP1)抗体联合抗腮腺分泌蛋白(parotid secretory protein, PSP)抗体在干燥综合征(Sj9gren’s syndrome, SS)诊断中的价值。方法:收集2020年1月至2022年12月就诊于河北医科大学第二医院风湿免疫科门诊及住院部的原发性SS(primary SS, pSS)患者60例,其他自身免疫病伴有口干和(或)眼干症状的患者30例为疾病对照组,体检中心的健康体检者30例为健康对照组,留取血清备用,同时记录一般资料、临床表现、实验室指标及其它辅助检查。采用2016年美国风湿病学会(American College of Rheumatology, ACR)/欧洲抗风湿病联盟(European League against Rheumatism, EULAR)干燥综合征分类标准作为pSS诊断金标准。采用化学发光免疫分析法检测免疫球蛋白G(immunoglobulin G,IgG)型抗SP1抗体和抗PSP抗体。用受试者工作特征(receiver operating characteristic, ROC)曲线评估抗SP1抗体和抗PSP抗体诊断pSS的准确性,并比较pSS组中抗SP1抗体和抗PSP抗体阳性患者与阴性患者的临床特征。采用t检验及Mann-Whitney U检验、方差分析、Kruskal-Wallis检验、卡方检验或Fisher确切概率法、Spearman相关分析进行统计学分析。结果:3组之间年龄(F=1.406,P=0.495)、性别(χ~2=2.105,P=0.349)差异无统计学意义。抗SP1抗体(H=16.73,P<0.001)和抗PSP抗体(H=26.09,P<0.001)在3组之间表达水平差异有统计学意义。组间比较发现,抗SP1抗体和抗PSP抗体表达水平在pSS和健康对照组之间差异均有统计学意义(P<0.001),抗PSP抗体表达水平在疾病对照组和健康对照组之间差异有统计学意义(P=0.009)。在pSS组、疾病对照组、健康对照组中,抗SP1抗体阳性率分别为58.33%vs. 40.00%vs.13.33%,差异有统计学意义(P<0.001);抗PSP抗体阳性率分别为75.00%vs. 56.17%vs.16.67%,差异有统计学意义(P<0.001)。抗SP1抗体、抗PSP抗体的曲线下面积分别为0.688(P<0.001)、0.720 (P<0.001),敏感性分别为58.33%(35Objective:To assess the diagnostic value of anti-salivary gland protein-1(SP1)antibody combined with anti-parotid secretory protein(PSP)antibody for Sj gren’s syndrome(SS).Methods:A total of 60 patients with primary SS(pSS)who were treated in the outpatient and inpatient department of Department of Rheumatology and Immunology of the Second Hospital of Hebei Medical University from January 2020 to December 2022 were collected.Thirty patients with other autoimmune diseases accompanied by dry mouth and/or dry eyes were collected as disease control group.Thirty healthy subjects from the physical examination center were collected for healthy control group,serum samples were obtained from all of them.Their general features and clinical information including clinical manifestations,laboratory examinations and other examinations were recorded.The 2016 American College of Rheumatology(ACR)/European League against Rheumatism(EULAR)classification criteria were adopted as the diagnostic standard of pSS.Immunoglobulin G(IgG)subtype of anti-SP1 antibody and anti-PSP antibody were detected by chemiluminescence immunoassay.The receiver operating characteristic(ROC)curve was used to evaluate the accuracy of anti-SP1 antibody and anti-PSP antibody in diagnosing pSS.The cli-nical characteristics of anti-SP1 antibody and anti-PSP antibody positive patients and negative patients in pSS group were further compared.Independent samples t test,Mann-Whitney U test,variance analysis,Kruskal-Wallis test,Chi-square test or Fisher’s exact test and Spearman correlation analysis were used for statistical analysis.Results:There was no significant difference in age(F=1.406,P=0.495)and gender(χ2=2.105,P=0.349)among pSS group,disease control group and healthy control group.The expression levels of anti-SP1 antibody(H=16.73,P<0.001)and anti-PSP antibody(H=26.09,P<0.001)were statistically different among the three groups.An intergroup comparison of anti-SP1 antibody expression levels showed that there was a statistically significant difference b

关 键 词：干燥综合征 抗唾液腺蛋白抗体 抗腮腺分泌蛋白抗体 

分 类 号：R593.2[医药卫生—内科学]