MiR-497通过下调XIAP诱导肝细胞癌LM3的凋亡  

MIR-497 INDUCES HEPATOCELLULAR CARCINOMA CELL LM3 APOPTOSIS THROUGH DOWNREGULATING XIAP

作　　者：刘溪涛[1] LIU Xitao(The Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010050

出　　处：《内蒙古医科大学学报》2024年第3期225-230,236,共7页Journal of Inner Mongolia Medical University

基　　金：内蒙古医科大学附属医院科研项目(NYFYYB 010)。

摘　　要：目的MiR-497在细胞行为中扮演着重要的角色,MiR-497可以与X连锁凋亡抑制蛋白(XIAP)3’-UTR相结合,共同在肝癌细胞的增殖和凋亡中起重要的作用。本研究探讨并验证MiR-497和XIAP在影响肝癌细胞增殖和细胞凋亡中的作用。方法选取2017年12月至2023年3月内蒙古医科大学附属医院75例原发性肝癌的患者作为研究对象。通过检测肝脏肿瘤组织和癌旁组织标本中MiR-497和XIAP的表达,将正常肝组织L02细胞与肝细胞癌组织LM3细胞中的MiR-497和XIAP水平进行比较。Ki-67蛋白表达及细胞凋亡通过流式细胞仪进行检测。MiR-497和XIAP之间的目标关系通过双荧光素酶报告基因检测进行验证分析。肝细胞癌组织中的LM3细胞可以被分为mimic NC,miR-97 mimic,si-NC,si-XIAP和miR-497 mimic+si-XIAP group。恶性细胞生长能力通过集落形成进行了评价。结果在肝细胞癌组织与癌旁组织对比中发现,MiR-497明显下调而XIAP的表达却明显增强。在肝癌组织中LM3细胞与正常肝组织中L02细胞相比中发现MiR-497下降而XIAP是升高的。肝癌组织中LM3细胞表现出细胞增殖增强和细胞凋亡减少的特性,并且在肝癌组织LM3细胞中MiR-497可以靶向调控XIAP基因。MiR-497显著的上调和/或XIAP显著降低都弱化了肝癌组织中LM3细胞的增殖和恶性生长,促使Caspase-3和Caspase-7酶活性升高,并促进细胞凋亡。结论在肝细胞癌组织与癌旁组织相比较中,MiR-497异常下调,而XIAP却是增强的。MiR-497也许可以通过靶向抑制XIAP的表达拮抗在Caspase酶活性中XIAP的抑制作用,从而促进肝癌细胞凋亡。Objective MiR-497 plays a significant role in cell behavior.Bioinformatics analysis showed that miR-497 can bind with the3-UTR of X-linked inhibitor of apoptosis protein(XIAP).This study investigated the role of miR-497 and XIAP in affecting hepatocellular carcinoma(HCC)cell proliferation and apoptosis.Methods There were 75 HCC patients between December 2017 and March 2023 in our hospital enrolled in this study.Tumor tissue and para-carcinoma tissue specimens were collected to test miR-497 and XIAP expression.MiR-497 and XIAP levels in normal liver L02 cells and HCC LM3 cells were compared.Ki-67 protein expression and cell apoptosis were detected by flow cytometry.The target relationship between miR-497 and XIAP was verified dual luciferase reporter assay.LM3 cells were divided into mimic NC,miR-497 mimic,si-NC,si-XIAP,and miR-497 mimic+si-XIAP group.Malignant cell growth ability was evaluated by colony formation.Results MiR-497 significantly downregulated,while XIAP expression obviously enhanced in HCC tissue compared with paracarcinoma tissue.MiR-497 reduced and XIAP elevated in LM3 cells compared with L02 cells.LM3 cells presented enhanced cell proliferation and reduced apoptosis.MiR-497 targeted regulated XIAP gene in LM3 cells.MiR-497 upregulation and/or XIAP reduction markedly weakened LM3 cell proliferation and malignant growth,elevated Caspase-3 and Caspase-7 enzyme activity,and promoted cell apoptosis.Conclusions MiR-497 abnormally downregulated,while XIAP enhanced in HCC tissue compared with para-carcinoma tissue.MiR-497 may antagonize the inhibitory effect of XIAP on Caspase enzyme activity through targeted suppressing XIAP expression,thus to promote HCC cell apoptosis.

关 键 词：MiR-497 XIAP 肝细胞癌 细胞凋亡 半胱天冬酶 

分 类 号：R274.9[医药卫生—中西医结合]