平衡透析法测定加米霉素与猪、牛和羊的血浆蛋白结合率  

Determination of Plasma Protein Binding Rates of Gamithromycin in Pig,Cattle and Sheep Using Equilibrium Dialysis Method

作　　者：姚鑫 叶昕熠 张鑫鑫[1,2] 符文真 刘雅红 周宇峰[1,2,3] YAO Xin;YE Xinyi;ZHANG Xinxin;FU Wenzhen;LIU Yahong;ZHOU Yufeng(State Key Laboratory for Animal Disease Control and Prevention,South China Agricultural University,Guangzhou 510642,China;College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物疫病防控全国重点实验室,广东广州510642 [2]华南农业大学兽医学院,广东广州510642 [3]岭南现代农业科学与技术广东省实验室,广东广州510642

出　　处：《中国兽医杂志》2024年第10期81-87,共7页Chinese Journal of Veterinary Medicine

基　　金：岭南现代农业实验室科研项目(NT2021006);国家自然科学基金项目(31902318);双一流学科建设和提升专项(2023B10564003)。

摘　　要：为测定加米霉素与常见家畜的血浆蛋白结合率(PPBR),本试验建立猪、牛和羊血浆中加米霉素的高效液相色谱-串联质谱检测方法,并对该方法的专属性、检测限、定量限、标准曲线、提取回收率和精密度进行考察。采用平衡透析法测定不同浓度(0.1、0.5和2.5μg/mL)加米霉素与猪、牛和羊的PPBR。结果显示,在考察浓度范围内,加米霉素在猪、牛和羊血浆中的专属性较好,检测限和定量限分别为0.5和1.0 ng/mL,标准曲线呈现良好的线性关系(r>0.997)。加米霉素在猪、牛和羊血浆中的提取回收率介于91.2%~107.8%,日内和日间精密度相对标准偏差(RSD)分别小于6.59%和9.16%。加米霉素与猪、牛和羊的平均PPBR分别为(21.86±4.19)%、(27.51±6.95)%和(19.64±3.58)%,且在考察的浓度范围内无浓度依赖特征。本试验所建方法准确可靠,可用于测定加米霉素与猪、牛和羊的PPBR。本试验结果可为加米霉素的药物代谢动力学研究及其在兽医临床中的科学用药提供参考依据。To determine the plasma protein binding rate(PPBR)of gamithromycin in common livestock,this study established a high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for detecting gamithromycin in the plasma of pigs,cattle,and sheep.The method's specificity,limit of detection,limit of quantification,standard curve,extraction recovery rate,and precision were evaluated.The PPBR of gamithromycin in pigs,cattle,and sheep was determined using equilibrium dialysis at different concentrations(0.1,0.5,and 2.5μg/mL).The results showed good specificity of gamithromycin in the plasma of pigs,cattle,and sheep within the concentration range studied,with limit of detection and limit of quantification of 0.5 and 1.0 ng/mL,respectively.The standard curves exhibited good linearity(r>0.997).The extraction recovery rates of gamithromycin in the plasma of pigs,cattle,and sheep ranged from 91.2%to 107.8%,with intra-day and inter-day precision relative standard deviations(RSD)of less than 6.59%and 9.16%,respectively.The average PPBR of gamithromycin in pigs,cattle,and sheep was 21.86%±4.19%,27.51%±6.95%,and 19.64%±3.58%,respectively,without concentration-dependent characteristics within the concentration range studied.The method developed in this study is accurate and reliable,making it suitable for determining the PPBR of gamithromycin in pigs,cattle,and sheep.The results of this study can serve as a reference for the pharmacokinetic research of gamithromycin and its scientific application in veterinary clinical practice.

关 键 词：加米霉素 血浆蛋白结合率 平衡透析法 高效液相色谱-串联质谱(HPLC-MS/MS) 

分 类 号：S859.7[农业科学—临床兽医学] R96[农业科学—兽医学]