人脐带间充质干细胞治疗多囊卵巢综合征模型小鼠卵巢组织中Rab27A的表达  

Expression of Rab27A in ovarian tissue of polycystic ovary syndrome model mice treated with human umbilical cord mesenchymal stem cells

作　　者：陶晨玥 陈帅 王丽萍 孟德芳 周东杰 周罗晶 Tao Chenyue;Chen Shuai;Wang Liping;Meng Defang;Zhou Dongjie;Zhou Luojing(School of Public Health,Yangzhou University,Yangzhou 225001,Jiangsu Province,China;College of Veterinary Medicine of Yangzhou University,Institute of Comparative Medicine of Yangzhou University,Yangzhou 225009,Jiangsu Province,China;Department of Biobank,Northern Jiangsu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225001,Jiangsu Province,China;Department of Rheumatology and Immunology,Northern Jiangsu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225001,Jiangsu Province,China;Department of Maternity,Northern Jiangsu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225001,Jiangsu Province,China;Department of Science and Technology,Northern Jiangsu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225001,Jiangsu Province,China)

机构地区：[1]扬州大学公共卫生学院,江苏省扬州市225001 [2]扬州大学兽医学院,扬州大学比较医学研究院,江苏省扬州市225009 [3]扬州大学附属苏北人民医院生物样本库,江苏省扬州市225001 [4]扬州大学附属苏北人民医院风湿免疫科,江苏省扬州市225001 [5]扬州大学附属苏北人民医院妇产科,江苏省扬州市225001 [6]扬州大学附属苏北人民医院科技处,江苏省扬州市225001

出　　处：《中国组织工程研究》2025年第25期5289-5295,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年项目(82001512),项目负责人:王丽萍;江苏省科技计划面上项目(BK20211118),项目负责人:王丽萍;江苏省卫生健康委医学科研面上项目(M2021044),项目负责人:王丽萍。

摘　　要：背景:多囊卵巢综合征是导致育龄女性不孕的常见生殖内分泌疾病,目前尚无有效的治疗方法,人脐带间充质干细胞可能有助于修复卵巢功能,但其治疗多囊卵巢综合征方面的研究较少。目的:探讨人脐带间充质干细胞治疗多囊卵巢综合征的作用及机制。方法:3周龄雌性ICR小鼠随机分为3组(n=15):正常对照组不作处理,模型组连续21 d灌胃来曲唑诱导建立多囊卵巢综合征模型,治疗组连续21 d灌胃来曲唑+经尾静脉注射人脐带间充质干细胞悬液。治疗前和治疗后7,14 d称量小鼠体质量;治疗后连续10 d阴道涂片检测小鼠动情周期;治疗后14 d,ELISA检测小鼠外周血性激素水平,苏木精-伊红染色观察卵巢形态学改变,免疫荧光、免疫组化检测卵巢组织中Rab27A蛋白表达。结果与结论:①与正常对照组相比,模型组小鼠动情周期紊乱,体质量明显升高,卵泡刺激素水平降低,黄体生成素和睾酮水平均升高,卵巢可见较多囊状扩张的卵泡,Rab27A蛋白表达水平降低;②与模型组相比,治疗组小鼠体质量降低,卵泡刺激素水平升高,黄体生成素和睾酮水平均降低,多囊状扩张的卵泡减少,Rab27A蛋白表达水平升高;③结果表明,人脐带间充质干细胞可能通过上调Rab27A的表达改善多囊卵巢综合征小鼠血清性激素水平及卵巢功能。BACKGROUND:Polycystic ovary syndrome is a common reproductive endocrine disease leading to infertility in women of reproductive age.Currently,there is no effective treatment.Human umbilical cord mesenchymal stem cells may help to repair ovarian function,but few studies have reported their role in the treatment of polycystic ovary syndrome.OBJECTIVE:To explore the effect and mechanism of human umbilical cord mesenchymal stem cells in the treatment of polycystic ovary syndrome.METHODS:3-week-old female ICR mice were divided into three groups(n=15).Normal control group was not treated.Model group was given letrozole for 21 days to induce polycystic ovary syndrome.Treatment group was given letrozole via intragastric administration for 21 consecutive days,and human umbilical cord mesenchymal stem cell suspension was injected through the tail vein.The body weight of mice was monitored before treatment,7 and 14 days after treatment.The estrous cycle of mice was detected by continuous vaginal smears for 10 consecutive days after treatment.Fourteen days after treatment,peripheral blood sex hormone levels of mice were detected by ELISA.Hematoxylin-eosin staining was used to observe ovarian morphological changes.Immunofluorescence and immunohistochemistry were used to detect the expression of Rab27A protein in ovarian tissues.RESULTS AND CONCLUSION:(1)Compared with the normal control group,the estrous cycle of mice in the model group was disturbed;body weight was significantly increased;follicle stimulating hormone was decreased;luteinizing hormone and testesterone were both increased;more follicles were found in the ovaries,and the relative expression level of Rab27A protein was decreased.(2)Compared with the model group,the treatment group had diminished body weight,increased follicle stimulating hormone,decreased luteinizing hormone and testesterone,decreased follicles with polycystic dilatation,and increased Rab27A protein relative expression level.(3)These findings suggest that human umbilical cord mesenchymal stem cel

关 键 词：人脐带间充质干细胞 多囊卵巢综合征 动情周期 卵巢功能 性激素 Rab27A 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R711.75