低氧暴露后大鼠外周血及骨髓红系的变化  

Changes in peripheral blood and bone marrow erythroblasts in rats after hypoxic exposure

作　　者：熊华[1] 李金杰 王璐 马婕[1] Xiong Hua;Li Jinjie;Wang Lu;Ma Jie(Qinghai University Affiliated Hospital(School of Clinical Medicine),Xining 810001,Qinghai Province,China)

机构地区：[1]青海大学附属医院(临床医学院),青海省西宁市810001

出　　处：《中国组织工程研究》2025年第25期5390-5395,共6页Chinese Journal of Tissue Engineering Research

基　　金：青海省科技厅自然科学基金项目(2020-ZJ-956Q),项目负责人:马婕。

摘　　要：背景:低氧暴露下大鼠外周血红细胞变化与骨髓有核红细胞增殖变化相关。低氧诱导因子、促红细胞生成素等相关调控因素参与其中。目的:探讨大鼠低氧暴露后外周血红细胞及骨髓有核红细胞的变化特点及相关因素。方法:模拟海拔5000 m的低压低氧环境建立实验大鼠模型,对照组大鼠饲养在海拔2260 m的实验室内,对比低氧暴露前后大鼠血常规、骨髓有核红细胞比例及低氧诱导因子、促红细胞生成素等相关指标。结果与结论:①实验组大鼠外周血红细胞计数、血红蛋白、红细胞压积等指标较对照组升高(P<0.05);②实验组大鼠骨髓CD71^(+)有核红细胞比例较对照组升高(P<0.05);③实验组大鼠骨髓CD71^(+)有核红细胞中低氧诱导因子2α表达水平较对照组升高(P<0.05);④实验组大鼠骨髓上清液促红细胞生成素水平较对照组升高(P<0.05)。结果表明,低氧暴露后大鼠外周血红细胞及骨髓有核红细胞比例增高,可能与骨髓有核红细胞中低氧诱导因子2α及骨髓上清液中促红细胞生成素水平增高有关。BACKGROUND:The changes in peripheral blood erythrocytes of rats exposed to hypoxia are related to changes in proliferation of erythroblasts in the bone marrow.Related regulatory factors such as hypoxia-inducible factor and erythropoietin are involved.OBJECTIVE:To explore the changes and related factors of peripheral blood erythrocytes and bone marrow erythroblasts in rats after hypoxic exposure.METHODS:An altitude of 5000 meters was simulated to establish an experimental rat model under a low-pressure and low-oxygen environment.The control group was raised in a laboratory at an altitude of 2260 meters.The blood routine,bone marrow erythroblast ratio,hypoxia-inducible factor,and erythropoietin of rats were compared before and after hypoxic exposure.RESULTS AND CONCLUSION:(1)The peripheral blood erythrocyte count,hemoglobin,hematocrit and other indicators of the experimental group rats were higher than those of the control group(P<0.05).(2)The proportion of bone marrow CD71+erythroblasts of the experimental group rats increased compared with the control group(P<0.05).(3)The expression level of hypoxia-inducible factor-2αin bone marrow CD71+erythroblasts of experimental group rats increased compared with the control group(P<0.05).(4)The erythropoietin level in the bone marrow supernatant of the experimental group rats was higher than that of the control group(P<0.05).The results indicate that the proportion of peripheral blood erythrocytes and bone marrow erythroblasts in rats increases after hypoxic exposure,which may be related to the increased levels of hypoxia-inducible factor-2αin bone marrow erythroblasts and erythropoietin in bone marrow supernatant.

关 键 词：低氧 大鼠 外周血 红细胞 骨髓 有核红细胞 

分 类 号：R459.9[医药卫生—治疗学] R331[医药卫生—临床医学] R599