干细胞抗卵巢颗粒细胞衰老的作用机制及进展  

Action mechanism and progress of stem cells against ovarian granulosa cell senescence

作　　者：林美玉 赵玺龙 高景 赵晶 阮光萍 Lin Meiyu;Zhao Xilong;Gao Jing;Zhao Jing;Ruan Guangping(Basic Medical Laboratory of 920 Hospital of Joint Logistics Support Force of Chinese PLA,Kunming 650032,Yunnan Province,China;Integrated Engineering Research Center of Cell Biological Medicine of State and Regions,Kunming 650032,Yunnan Province,China;Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province,Kunming 650032,Yunnan Province,China)

机构地区：[1]解放军联勤保障部队第九二〇医院基础医学实验室,云南省昆明市650032 [2]干细胞与免疫细胞生物医药技术国家地方联合工程研究中心,云南省昆明市650032 [3]云南省细胞治疗技术转化医学重点实验室,云南省昆明市650032

出　　处：《中国组织工程研究》2025年第25期5414-5421,共8页Chinese Journal of Tissue Engineering Research

基　　金：云南省科技计划项目重大科技专项(2018ZF007),项目负责人:潘兴华;云南省重点项目(202101AS070039),项目负责人:阮光萍;中国人民解放军联勤保障部队第九二〇医院院管课题(2020YGD12),项目负责人:阮光萍。

摘　　要：背景:干细胞疗法作为一种新兴的治疗手段,有望改善卵巢环境,延缓卵巢颗粒细胞的衰老,为高龄妊娠女性带来新的希望。目的:综述干细胞抗卵巢颗粒细胞衰老的机制及研究进展。方法:检索中国知网和PubMed数据库中关于干细胞抗卵巢颗粒细胞衰老的文献,以“干细胞,卵巢颗粒细胞,衰老”为中文检索词,以“stem cells,ovary granulosa cells,aging”为英文检索词,最终纳入67篇文献进行分析。结果与结论:①总结了5种卵巢颗粒细胞衰老的机制:DNA损伤和修复能力下降、氧化应激、线粒体功能受损、炎症反应、激素水平变化;②整理了当前干细胞治疗卵巢颗粒细胞衰老的5种机制:促进增殖和抑制凋亡、分化潜能及再生能力、分泌因子、抗氧化、抗炎症反应和免疫调节;③目前有多种类型的干细胞可通过多种方式协同治疗卵巢颗粒细胞衰老,干细胞疗法在恢复女性生育能力方面具有广阔前景。BACKGROUND:As an emerging treatment method,stem cell therapy is expected to improve the ovarian environment,delays the aging of ovarian granulosa cells,and brings new hope to older pregnant women.OBJECTIVE:To review the mechanism and research progress of stem cells against ovarian granulosa cell senescence.METHODS:CNKI and PubMed databases were retrieved for articles on stem cell therapy for ovarian granulosa cell senescence.Chinese and English search terms were“stem cells,ovary granulosa cells,aging.”Finally,67 articles were included for analysis.RESULTS AND CONCLUSION:(1)This review summarizes five mechanisms of ovarian granulosa cell senescence:DNA damage and decreased repair capacity,oxidative stress,impaired mitochondrial function,inflammatory response,and changes in hormone levels.(2)This article summarizes five main mechanisms of stem cells in the treatment of ovarian granulosa cell senescence:promoting proliferation and inhibiting apoptosis,differentiation potential and regeneration ability,secreting factors,anti-oxidation,anti-inflammatory response,and immune regulation.(3)At present,there are various types of stem cells that can cooperate to treat ovarian granulosa cell senescence through a variety of ways,and stem cell therapy has broad prospects in restoring female fertility.

关 键 词：干细胞 卵巢颗粒细胞 衰老 机制 氧化应激 抑制凋亡 细胞因子 抗氧化 抗炎症反应 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R394.2