小檗碱的体外抗衣原体活性研究  

The antichlamydial activity of berberine in vitro

作　　者：解文霞 朱怡昕 包小峰 XIE Wenxia;ZHU Yixin;BAO Xiaofeng(School of Pharmacy,Nantong University,Nantong 226001,China)

机构地区：[1]南通大学药学院,南通226001

出　　处：《生物学杂志》2024年第5期1-5,共5页Journal of Biology

基　　金：国家自然科学基金项目(31400165);江苏省高等学校基础科学(自然科学)研究重大项目(23KJA310004);南通市基础科学研究计划项目(JC22022043);南通市第六期江海英才市级培养专项。

摘　　要：研究旨在阐明天然产物小檗碱的体外抗衣原体活性。首先,通过测定小檗碱对沙眼衣原体L2和鼠衣原体MoPn包涵体形成数量和感染性子代滴度的影响,发现小檗碱对两种衣原体均呈浓度依赖性抑制。其次,采用CCK-8试剂盒测定化合物对细胞活力的影响,显示浓度不高于8μmol/L时小檗碱无明显宿主细胞毒性。借助宿主细胞和衣原体预处理实验,发现小檗碱不能直接作用于宿主细胞,仅能微弱地直接作用于衣原体而影响衣原体的感染过程。最后,分析小檗碱对衣原体增殖过程的影响。后加药和撤药实验结果显示,小檗碱主要作用于2~12 h的EB→RB转化阶段;长期耐受实验结果表明,小檗碱可以延缓衣原体的生长周期,但不能完全杀死衣原体。综上,小檗碱具有广谱的体外抗衣原体活性,对衣原体增殖过程的干扰作用强于对感染过程的影响。The aim of this study was to elucidate the antichlamydial activity and mechanism of the natural product berberine in vitro.Firstly,the antichlamydial activity of berberine was evaluated.The data showed that berberine inhibited the formation of inclusion body and the titer of infectious progenies in a dose-dependent manner for both Chlamydia trachomatis L2 and Chlamydia muridarum MoPn.Secondly,host cell viability was measured with CCK-8 kit to evaluate the cytotoxicity of the compound.No significant host cytotoxicity was observed when berberine concentration was not higher than 8μmol/L.Then,the effect of berberine on the chlamydial infection process was examined by the pretreatment of compound with host cells or Chlamydia.Pretreatment assays showed that berberine could weakly directly act on Chlamydia but not host cells to disturb the chlamydial infection process.Finally,the effect of berberine on the chlamydial proliferation process was analyzed.Later treatment and withdrawal assay revealed that the EB→RB transformation stage at 2-12 h of chlamydial period is the mainly target for berberine.The long-term tolerance assay displayed that berberine delayed the chlamydial development cycle,but could not kill it completely.In conclusion,berberine has a broad-spectrum antichlamydial activity in vitro,and its inhibitory effect on the proliferation period was stronger than that on the infection process.

关 键 词：衣原体 小檗碱 抗衣原体活性 感染过程 增殖过程 

分 类 号：R966[医药卫生—药理学]