不同种植年限薰衣草根系分泌物研究  

Study and analysis of root exudates of Lavandula angustifolia at different cultivation years

作　　者：师仁增 邓霞 王君竹 柳雯雯 李桂花 郭子轩 焦子伟 SHI Renzeng;DENG Xia;WANG Junzhu;LIU Wenwen;LI Guihua;GUO Zixuan;JIAO Ziwei(School of Biological Sciences and Technology,Yili Normal University,Yining 835000,China)

机构地区：[1]伊犁师范大学生物科学与技术学院,伊宁835000

出　　处：《生物学杂志》2024年第5期61-69,共9页Journal of Biology

基　　金：国家自然科学基金项目(32160312);新疆维吾尔自治区重点研发项目(2022B02021-2);学实高层次人才岗位项目(YLSDXSDTR22001)。

摘　　要：为探究不同种植年限对新疆伊犁薰衣草根系分泌物的影响,采用液相色谱-质谱联用仪(LC-MS)对种植1年、3年和5年薰衣草(L1、L3和L5)的根际土壤,以及未种植薰衣草(L0)的土壤进行非靶向代谢组分析。结果表明,根系分泌物包括有机酸类、脂类、核酸类、碳水化合物类、维生素类和辅助因子类、激素类、多肽类、抗生素类和类固醇类。相较L0,L1、L3、L5分别筛选出26、17与21种显著差异分泌物(P<0.001);L1、L3和L5分别富集到13、10条和9条显著差异代谢通路(P<0.05)。相较L1,L3和L5分别筛选到27种(P<0.05)和9种显著差异分泌物(P<0.001);L3和L5分别富集到4条和9条显著差异代谢通路(P<0.05)。与L3相比,L5筛选到13种显著差异分泌物(P<0.05),富集到6条显著代谢通路(P<0.05)。总体来说,随着种植年限的延长,甾醇类物质先下降后上升,维生素类、多肽类和核苷酸类逐渐下降,薰衣草根系分泌物发生显著变化。To explore the impact of different cultivation periods on the exudates of Lavandula angustifolia in Yili,Xinjiang,non-targeted metabolomic analysis was conducted by using liquid chromatography-mass spectrometry(LC-MS)on the rhizosphere soil samples of 1-year-old,3-year-old,and 5-year-old lavender plants(named L1,L3,and L5),as well as soil samples without lavender cultivation(L0).The results indicated that root exudates included nine main categories:organic acids,lipids,nucleic acids,carbohydrates,vitamins and cofactors,hormones,peptides,antibiotics,and steroids.Compared to L0,L1,L3,and L5 resulted in the identification of 26,17,and 21 significantly different exudates,respectively(P<0.001).Additionally,L1,L3,and L5 exhibited enrichment in 13,10,and 9 significantly different metabolic pathways(P<0.05),respectively.Compared to L1,L3 and L5 showed 27(P<0.05)and 9 significantly different exudates(P<0.001),respectively,and enrichment in 4 and 9 significantly different metabolic pathways(P<0.05),respectively.Furthermore,when compared to L3,L5 presented 13 significantly different exudates(P<0.05)and enrichment in 6 significantly different metabolic pathways(P<0.05).Overall,the exudates of Lavandula angustifolia root system underwent significant changes as the cultivation period extended,with sterols initially decreasing and then increasing,while vitamins,peptides,and nucleotides gradually decreased.

关 键 词：根系分泌物 非靶向代谢组 薰衣草 连续种植 根际 

分 类 号：Q945[生物学—植物学]