拉曼光谱技术在角膜组织成分检测中的应用  

Applications of Raman Spectroscopy Technology in Corneal Tissue Composition Detection

作　　者：郭智君 马苗立 卢思超 郭前进 杨强 Guo Zhijun;Ma Miaoli;Lu Sichao;Guo Qianjin;Yang Qiang(Academy of Artificial Intelligence,Beijing Institute of Petrochemical Technology,Beijing 102617,China;Beijing Academy of Safety Engineering and Technology,Beijing 102617,China;School of Information Engineering,Beijing Institute of Petrochemical Technology,Beijing 102617,China)

机构地区：[1]北京石油化工学院人工智能研究院,北京102617 [2]北京市安全生产工程技术研究院,北京102617 [3]北京石油化工学院信息工程学院,北京102617

出　　处：《中国激光》2024年第15期124-134,共11页Chinese Journal of Lasers

基　　金：国家自然科学基金(52361145714,62303064);北京石油化工学院致远基金(2023015,2023007);北京市教育委员会专项项目(22019821001);北京石油化工学院人工智能青年科学家攀登计划(BIPTAAI-2023001)。

摘　　要：角膜作为眼球的最外层组织,对于维持眼球结构和视力至关重要。然而,在日常用眼过程中,角膜易遭受创伤和感染,从而损害视力。目前常用的裂隙灯显微镜检查、光学相干层析成像及共聚焦显微镜检查等基于形态结构的角膜检测技术无法给出组织成分信息。拉曼光谱技术由于可以在不破坏组织结构的基础上获取生物分子信息,在角膜组织成分检测中具有重要的应用价值。本文从拉曼光谱原理出发,综述了其在角膜组织成分检测中的应用,最后总结并展望了其发展趋势。Significance The cornea is the outermost tissue of the eye,and it is crucial for maintaining the structure and vision of the eye.However,everyday activities can expose the cornea to trauma-associated injuries and infections that may compromise vision.Common corneal detection techniques,such as slit lamp imaging,optical coherence tomography,and confocal microscopy,are based on a morphological structural analysis of the physiological status of the corneal tissue.However,they cannot provide information on tissue composition.Raman spectroscopy can obtain biomolecular information without damaging the tissue structure and has important application value in the detection of corneal tissue composition.Progress Analysis of the hydration state of the cornea using Raman spectroscopy can be used to evaluate tissue function and vitality.In 1995,Siew et al.characterized the degree of corneal hydration using the ratio of the intensity integral of the O—H of water to the intensity integral of the C—H of the protein in the Raman spectrum,I_(OH)/I_(CH).In 1998,Bauer et al.determined a positive correlation between the degree of hydration and distance from the corneal stroma to the tear film.Bauer et al.and Erckens et al.observed that the value of I_(OH)/I_(CH) decreased as the cornea was treated with dehydrating agent drugs in subsequent research studies.In 2003,Fisher et al.determined that I_(OH)/I_(CH) of a bovine cornea treated with lamellar keratectomy decreased faster than that of a manually debrided cornea when the cornea was subjected to forced flow drying.The distribution and content of biomolecules in the cornea change during corneal development,aging,decellularization,and corneal cross-linking(CXL)processes.In 2012,Pang et al.analyzed the changes in the vibrational patterns of proteins,amino acids(tyrosine,proline,phenylalanine,and valine),and protein secondary structures(amide I and amide II)in embryonic chicken corneas over 18 days of development.In 2010,Yamamoto et al.determined that solar radiation causes fragment

关 键 词：角膜 组织成分 拉曼光谱 生物分子 

分 类 号：O433[机械工程—光学工程]