榆林市绒山羊源大肠埃希氏菌耐药基因分子流行病学调查  

Molecular Epidemiological Investigation of Drug Resistance Genes in E.coli of Cashmere Goats in Yulin City

作　　者：郭志伟 王欣 郝建新 吴瑞瑞 罗逸青 刘万华 陈占莉 屈雷[1] 窦永喜[4] 翟军军 GUO Zhi-wei;WANG Xin;HAO Jian-xin;WU Rui-rui;LUO Yi-qing;LIU Wan-hua;CHENG Zhan-li;QU Lei;DOU Yong-xi;ZHAI Jun-jun(Shaanxi Province Engineering&Technology Research Center of Cashmere Goats,Yulin University,Yulin,Shaanxi 719000,China;College of Life Sciences,Yulin University,Yulin,Shaanxi 719000,China;Yulin City Key Laboratory of Veterinary Public Health and Biosafety,Yulin,Shaanxi 719000,China;State Key Laboratory of Animal Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730046,China;Yulin Animal Husbandry and Veterinary Service Center,Yulin,Shaanxi 719000,China)

机构地区：[1]榆林学院陕西省绒山羊工程技术研究中心,陕西榆林719000 [2]榆林学院生命科学学院,陕西榆林719000 [3]榆林学院榆林市兽医公共卫生与生物安全重点实验室,陕西榆林719000 [4]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [5]榆林市畜牧兽医服务中心,陕西榆林719000

出　　处：《动物医学进展》2024年第11期119-124,共6页Progress In Veterinary Medicine

基　　金：陕西省创新能力支撑计划项目(2021PT-017);国家自然科学基金地区科学基金项目(31960710,32060810);陕西省重点研发计划项目(2022NY-100)。

摘　　要：为了解榆林市绒山羊大肠埃希氏菌对抗菌药物的耐药性以及耐药基因的携带情况,采集榆林地区羔羊、育成羊、经产母羊与种公羊粪便样品各72份,通过药敏试验检测其耐药表型,PCR扩增检测其耐药基因携带情况。试验共分离到288株大肠埃希氏菌,药敏试验结果表明,榆林市羊源大肠埃希氏菌对检测的16种抗菌药物均有不同程度的耐药性,耐药率较高的为林可霉素100%(288/288)、青霉素97.57%(281/288)、克林霉素96.88%(279/288)、红霉素93.06%(268/288),其中96.88%(276/288)为多重耐药,以3~4重耐药为主。耐药基因检出率较高的为gyrA、TEM、gyrB、CTX-M 9、TEM-1,分别占99.31%(286/288)、97.57%(281/288)、94.44%(272/288)、88.19%(254/288)、81.60%(235/288)。经统计学分析,耐药基因检出数在羔羊、育成羊、经产母羊与种公羊之间无显著差异。分离的288株大肠埃希氏菌抗菌药物耐药普遍,耐药基因分布广泛。试验结果为榆林市绒山羊抗菌药物的科学使用提供了基础数据,同时也为榆林市公共卫生安全提供了理论支撑。To investigate the antimicrobial resistance of Escherichia coli of Cashmere goats in Yulin and the carrying of drug resistance genes,72 fecal samples were collected from lambs,growing goats,multiparous ewes and rams in Yulin area,and their drug resistance phenotypes were detected by drug susceptibility test,and drug resistance genes were detected by PCR amplification.A total of 288 strains of Escherichia coli were isolated in this experiment.The results of drug susceptibility test showed that Escherichia coli from goats in Yulin had different degrees of resistance to the 16 antimicrobials detected.The high drug resistance rates were lincomycin 100%(288/288),penicillin 97.57%(281/288),clindamycin 96.88%(279/288),erythromycin 93.06%(268/288),among which 96.88%(276/288)were multiple drug resistance,mainly 3-4 drug resistance.The high detection rates of drug resistance genes were gyrA,TEM,gyrB,CTX-M 9 and TEM-1.They accounted for 99.31%(286/288),97.57%(281/288),94.44%(272/288),88.19%(254/288)and 81.60%(235/288),respectively.By statistical analysis,there was no significant difference in the number of drug resistance genes detected among lambs,growing goats,multiparous ewes and rams.Antimicrobial resistance was common in 288 strains of Escherichia coli isolated in this study,and drug resistance genes were widely distributed.This experiment provided basic data for the scientific use of antimicrobials in Cashmere goats in Yulin,and also provided theoretical support for public health safety in Yulin.

关 键 词：大肠埃希氏菌 抗菌药物 耐药性 绒山羊 

分 类 号：S852.612[农业科学—基础兽医学] S858.27[农业科学—兽医学]