Box-Behnken优化红花多糖提取工艺研究  

Study on Box-Behnken for optimization of extraction of polysaccharides from Carthamus tinctorius L.

作　　者：王福玲 WANG Fuling(School of Pharmacy,Institute of Cell and Molecular Biology,Harbin University of Commerce,Harbin 150076,China;Heilongjiang Province Key Laboratory of Drug Prevention and Treatment for Senile Diseases,Harbin 150076,China;Zhejiang Shangyao Jiuxu Pharmaceutical Co.,Ltd.,Jinhua 321016,China)

机构地区：[1]哈尔滨商业大学药学院细胞与分子生物学研究所,哈尔滨150076 [2]黑龙江省预防与治疗老年性疾病药物研究重点实验室,哈尔滨150076 [3]浙江上药九旭药业有限公司,浙江金华321016

出　　处：《哈尔滨商业大学学报（自然科学版）》2024年第5期523-528,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：哈尔滨商业大学教学改革与教学研究一般项目(HSDJY202118)。

摘　　要：采用单因素和Box-Behnken响应面法优化实验条件,确定红花多糖提取的最佳条件.红花多糖的最佳提取工艺参数为:料液比1∶20(g/mL)、提取时间90 min、提取温度90℃、粉碎度0.600~0.125 mm,此条件下多糖得率为38.27%.因素的显著性顺序是粉碎度>提取温度>提取时间>料液比.红花多糖可作为一种新型抗氧化药物及食物开发.The single factor and Box-Behnken response surface method were used to optimize the experimental conditions.To determine the best conditions for extraction of polysaccharides from Carthamus tinctorius L.The best technological parameters for extraction of polysaccharides were ratio of solid to liquid of 1∶20(g/mL),extraction time of 90 min,extraction temperature of 90℃,and crushing degree of 0.600~0.125 mm.Under the conditions,the yield of polysaccharides was up to 38.27%.A variety of factors affected yield of polysaccharides significant performance crushing degree>extraction temperature>extraction time>liquid ratio.Safflower polysaccharides can be used as a new type of antioxidant drug and food development.

关 键 词：红花多糖 工艺优化 单因素 响应面法 实践探索 

分 类 号：R284.2[医药卫生—中药学]