心室辅助装置溶血测试中游离血红蛋白检测方法比较  

Comparative of detection method of free hemoglobin in ventricular assist device hemolysis test

作　　者：葛婉宁 梅旭 张柳笛 GE Wanning;MEI Xu;ZHANG Liudi(Artificial Organ Technology Laboratory,Bio-manufacturing Research Centre,School of Mechanical and Electrical Engineering,Soochow University,Suzhou,Jiangsu Province 215131;State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096)

机构地区：[1]苏州大学机电工程学院,生物制造研究中心,人工器官研究所,江苏苏州215131 [2]东南大学生物科学与医学工程学院,生物电子学国家重点实验室,南京210096

出　　处：《北京生物医学工程》2024年第5期500-505,共6页Beijing Biomedical Engineering

基　　金：国家自然科学基金青年基金(31700817);第62批国家博士后基金(2017M620224)资助。

摘　　要：目的评价溶血测试中游离血红蛋白检测的三种方法,得出一种最适合于心室辅助装置的方法。方法通过对比研究,在阐述联苯胺法、Harboe测试法和Cripps测试法测试血红蛋白含量的原理和方法基础上,对不同浓度的标准血红蛋白样本进行测试,描绘出血红蛋白标准液浓度与吸光度关系的标准曲线,对比分析三种方法在实验操作和结果差异性方面的优缺点。与此同时,对比1%和0.2%邻甲联苯胺溶液对联苯胺法的影响,并分析Harboe法中不同浓度稀释剂的影响(0.01%的碳酸钠溶液和0.1%的碳酸钠溶液)结果Cripps法实验重复性差,拟合系数较低(R^(2)=0.8019),并不能得到优质的标准曲线。联苯胺法和Harboe法实验重复性好,可以得到拟合效度高的标准曲线(R^(2)均大于0.99)。联苯胺法推荐使用1%邻甲联苯胺溶液,Harboe法中,0.01%的碳酸钠溶液更适用。但联苯胺法对试剂的要求很严格,要现配现用,并且测量结果对时间有依赖性。结论与其他测试方法相比,Harboe法可以弥补上述两种方法的不足,适用于实验室快速自动化分析,更适用于心室辅助装置的溶血测试。Objective To evaluate three methods for detecting free hemoglobin in hemolysis tests and determine the most suitable method for ventricular assist devices.Methods By conducting a comparative study,we describe the principles and methods of the Benzidine,Harboe,and Cripps methods for hemoglobin content testing.Standard hemoglobin samples of different concentrations were tested to plot standard curves depicting the relationship between hemoglobin concentration and absorbance.The advantages and disadvantages of the three methods were analyzed regarding experimental operation and result variability.Additionally,the impact of 1%and 0.2%ortho-tolidine solutions on the Benzidine method and the effect of different diluent concentrations(0.01%sodium carbonate solution and 0.1%sodium carbonate solution)in the Harboe method were compared.Results The Cripps method showed poor repeatability with a low fitting coefficient(R^(2)=0.8019)and failed to produce a high-quality standard curve.The Benzidine and Harboe methods exhibited good repeatability and could yield high-fitting standard curves(both with R^(2)>0.99).The Benzidine method is recommended to use a 1%ortho-tolidine solution,and the Harboe method is more suitable with a 0.01%sodium carbonate solution.However,the Benzidine method requires strict reagent preparation and immediate use,with time-dependent measurement results.Conclusions Compared to other testing methods,the Harboe method can overcome the shortcomings of the other two methods.It is suitable for rapid automated analysis in laboratories and is more appropriate for hemolysis testing in ventricular assist devices.

关 键 词：心室辅助装置 血浆游离血红蛋白 联苯胺法 Harboe法 Cripps法 

分 类 号：R318.04[医药卫生—生物医学工程]