外源肌肽对干旱胁迫‘师稻960’水稻幼苗生长的缓解效应  

Mitigating effect of exogenous l-carnosine on the growth of‘Shidao 960’rice seedlings under drought stress

作　　者：魏红宇 宋子慧 何珊 梁慧苑 余昆驰 简伟 彭春霖 刘艳 韩佳佳 赵正武 WEI Hongyu;SONG Zihui;HE Shan;LIANG Huiyuan;YU Kunchi;JIAN Wei;PENG Chunlin;LIU Yan;HAN Jiajia;ZHAO Zhengwu(College of Life Sciences,Chongqing Engineering Research Center of Specialty Crop Resources,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学生命科学学院/重庆市特色作物资源工程技术研究中心,重庆401331

出　　处：《植物生理学报》2024年第8期1280-1290,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31670326);农业农村部农作物精准鉴定项目(19230586);重庆市技术创新与应用发展重点专项(CSTB-2022TIAD-KPX0015和CSTB2022TIAD-KPX0018)。

摘　　要：干旱胁迫是限制作物生长的重要非生物因素之一。为探究干旱胁迫下外源施加肌肽对水稻(Oryza sativa)幼苗生长的影响,以‘师稻960’为材料,对干旱胁迫幼苗用不同浓度肌肽处理,研究幼苗表型、生理和分子水平上的变化,并用灰色关联度和相关性分析,揭示不同浓度肌肽和干旱胁迫幼苗表型与生理的关系。结果表明:外源施加肌肽可以增强幼苗过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,使渗透调节物质尤其是脯氨酸(Pro)含量增加,丙二醛(MDA)、过氧化氢(H_(2)O_(2))、超氧阴离子(O_(2)^(·-))含量和相对电导率(REC)相对单独干旱处理明显下降,同时诱导水稻干旱胁迫响应相关基因(Os P5CS、WDL1、Os MPK1、Os DREB1A、Os LEA3-1、Os SCL30)表达,从而缓解干旱对幼苗的损伤。外源施加肌肽浓度过低效果不明显,过高加重幼苗损伤,最适浓度为1 mmol·L^(-1)。Drought stress is one of the important abiotic factors restricting crop growth.In order to explore the effect of exogenous l-carnosine on the growth of rice(Oryza sativa)seedlings under drought stress,‘Shidao 960’was used as the material.Drought-stressed seedlings were treated with different concentrations of l-carnosine to study the changes of seedling on phenotype,physiology and molecular levels.Gray correlation and correlation analysis were used to reveal the relationship between different concentrations of l-carnosine and the phenotype and physiology of drought-stressed seedlings.The results show that exogenous l-carnosine could enhance the activities of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and ascorbate peroxidase(APX)in seedlings.The contents of osmoregulatory substances,especially proline(Pro),were increased,while the contents of malondialdehyde(MDA),hydrogen peroxide(H_(2)O_(2))and superoxide anion(O_(2)^(·-))and relative electrical conductivity(REC)were obviously decreased compared with individual drought treatment.At the same time,the expression of rice drought-stress-response-related genes(OsP5CS,WDL1,OsMPK1,OsDREB1A,OsLEA3-1,and OsSCL30)was induced,thereby alleviating the drought damage to seedlings.Too low concentration of exogenous l-carnosine had no obvious effect,but too high concentration aggravated seedling injury,and the optimum concentration is 1 mmol·L^(-1).

关 键 词：肌肽 干旱胁迫 水稻幼苗 基因表达 

分 类 号：S511[农业科学—作物学]