冬小麦茎鞘果聚糖代谢对干旱胁迫响应及与抗旱性关系  

Response of fructan metabolism in winter wheat stem to drought stress and its relationship with drought resistance

作　　者：寇一泓 李延坤 孟鈺 余维宝 徐铖堂 韩守威 李升东[1] 王宗帅[1] 司纪升[1] 张宾[1] 孔令安[1] 王法宏[1] 李华伟[1] KOU Yihong;LI Yankun;MENG Yu;YU Weibao;XU Chengtang;HAN Shouwei;LI Shengdong;WANG Zongshuai;SI Jisheng;ZHANG Bin;KONG Lin’an;WANG Fahong;LI Huawei(Crop Research Institute of Shandong Academy of Agricultural Sciences/National Engineering Research Center for Wheat and Corn/Shandong Wheat Technology Research Center,Jinan 250100,China;Shandong Seed Management Station,Jinan 250100,China;College of Agriculture,China Agricultural University,Beijing 100193,China)

机构地区：[1]山东省农业科学院作物研究所/小麦玉米国家工程研究中心/山东省小麦技术研究中心,济南250100 [2]山东种子管理总站,济南250100 [3]中国农业大学农学院,北京100193

出　　处：《植物生理学报》2024年第8期1339-1347,共9页Plant Physiology Journal

基　　金：泰山产业领军人才项目(tscy20190106);国家重点研发计划项目“山东冬小麦产量与效率层次差异形成机制与丰产增效途径”(2016YFD0300105-5);国家小麦产业技术体系(CARS-03-22)。

摘　　要：2017~2019年在盆栽条件下,以抗旱型小麦品种‘济麦22’(‘JM22’)和水分敏感型品种‘临麦2号’(‘LM2’)为材料,研究了挑旗至成熟期干旱对茎鞘贮藏糖代谢的影响及其与植株抗性和产量的关系。结果表明,干旱使‘JM22’和‘LM2’产量分别下降12.8%和36.6%,提高了小麦茎鞘贮藏物质对籽粒的贡献率;胁迫2~5 d后,SST和FFT活性受到抑制,FEH活性提高,DP≥4果聚糖和可溶性总糖含量下降,但DP=3果聚糖含量上升;胁迫后5~10 d茎鞘内糖含量与对照差异变小,‘JM22’糖含量提高值大于‘LM2’;干旱提高了‘JM22’胁迫后15~31 d茎鞘内SST和FFT活性,促进了DP≥4和DP=3果聚糖的积累。干旱提高了两品种灌浆后期FEH活性,促进了果聚糖的转化输出。干旱发生后旗叶内的H_(2)O_(2)和O_(2)^(-)的积累量,MDA含量,SOD和CAT活性短时间内快速升高,但随胁迫时间的延长而下降;加速了灌浆后期旗叶H_(2)O_(2)和O_(2)^(-)的积累,MDA含量,SOD和CAT活性地升高。Under potted condition,two winter wheat cultivars,the drought-resistant wheat variety,‘Jimai 22’(‘JM22’),and the water-sensitive wheat variety,‘Linmai 2’(‘LM2’),were chosen to study the effects of drought stress on the metabolism of fructose in wheat stem and sheath from flag leaf stage to ripening stage,and its relationship with drought resistance and grain yield.The results showed drought decreased the yields of‘JM22’and‘LM2’by 12.8%and 36.6%,and increased the contribution rate of the dry matter stored in the stem and sheath to the grains of the wheats.During the 2nd to 5th day of drought stress,sucrose:sucrose fructosyltransferase(SST)and fructan:fructan fructosyltransferase(FFT)activity of the stem and sheath were inhibited,while fructan exohydrolase(FEH)activity was increased.At the same time,the content of fructose with polymerization(DP)≥4 fructose,and total water-soluble sugar(WSC)decreased,but the content of fructose with DP=3 increased;During the 5th to 10th day of drought stress,the contents of fructan and WSC in the stem and sheath were recovered,but the recovery magnitude of‘JM22’was much greater than that of‘LM2’.During the 15th to 31st day of drought resistance,drought enhanced the activity of SST and FFT in the stem and sheath of‘JM22’,which promoted the accumulation of fructose with DP≥4 and DP=3.The activity of FEH of both wheat cultivars were much higher under drought condition than control during in the later stage of grain filling,which benefited the transform and output of fructan.The accumulation of H_(2)O_(2)and O_(2)^(-),content of MDA,the activity of SOD and CAT in wheat flag leaf increased rapidly at beginning of drought resistance,but they gradually recovered as the drought resistance persisted.The accumulation of H_(2)O_(2)and O_(2)^(-),the content of MDA,and the activity of SOD and CAT were enhanced under drought resistance at the late stage of grain filling.

关 键 词：干旱 贮藏糖类 再转运 抗旱性 产量 

分 类 号：S512.11[农业科学—作物学]