水稻粒长基因GW7的分子标记开发与利用  

Development and Application of Molecular Marker for Grain Length Gene GW7 in Rice(Oryza sativa)

作　　者：郑国利 顾巧美 杜明 方玉 王攀[1] ZHENG Guo-Li;GU Qiao-Mei;DU Ming;FANG Yu;WANG Pan(Shanghai ZKW Molecular Breeding Technology Co.,Ltd.,Shanghai 200030,China;Anhui Win-all Hi-tech Seed Co.,Ltd.,Hefei 230088,China)

机构地区：[1]上海中科荃银分子育种技术有限公司,上海200030 [2]安徽荃银高科种业股份有限公司,合肥230088

出　　处：《农业生物技术学报》2024年第10期2218-2227,共10页Journal of Agricultural Biotechnology

摘　　要：水稻(Oryza sativa)粒长是重要的农艺性状,与水稻产量和稻米外观品质密切相关。为进一步挖掘改良水稻粒长新的分子标记,本研究在前人克隆的基础上,根据GW7基因(Gene ID:10208)在功能区域上发生的单碱基突变,结合五引物扩增受阻突变体系(penta-primer amplification refractory mutation system,PARMS)技术,开发了GW7基因荧光分子标记。结合粒长表型及PCR测序,对该标记在13个水稻亲本材料的检测结果表明,该标记可以准确鉴定出不同的GW7基因型。之后,利用该标记从94份水稻材料中筛选到了16份纯合GW7基因型的材料;分子标记辅助选择的结果表明,在'R91'和'卓20'的F2代中,可以获得粒长增加的优良水稻材料。本研究所选标记能够在苗期筛选目标单株,开花期进行杂交、回交,无需等到成熟收获,从而加快水稻外观品质的育种进程。Rice(Oryza sativa)grain length is an important agronomic trait,which is closely related to rice yield and appearance quality.In order to further explore new molecular markers for improving rice grain length,this study developed a fluorescent molecular markers of GW7(Gene ID:10208)gene based on the single base mutation of GW7 gene in the functional region,combined with penta-primer amplification refractory mutation system(PARMS).Combined with grain length phenotype and PCR product sequencing,the detection results of the marker in 13 rice parent materials showed that the marker could accurately identify different GW7 genotypes.Then,16 homozygous GW7 genotypes were selected from 94 rice materials by using this marker.The results of marker-assisted selection showed that excellent rice materials with increased grain length could be obtained in F2 generation of'R91'and'zhuo20'.The marker this study developed can help to selecte individual plants with target traits at the seedling stage,and perform cross-breeding and backcrossing during the flowering period,without having to wait until maturity and harvest thereby accelerating the breeding process for rice grain quality traits.

关 键 词：水稻 粒长 五引物扩增受阻突变体系(PARMS) 荧光分子标记 GW7 

分 类 号：S336[农业科学—作物遗传育种] S338[农业科学—农艺学]